[ PROPERTIES ]
Source: Prokaryotic expression
Host: E.coli
Residues: Leu99~Cys216
Tissue Specificity: Heart, Lung, Spleen.
Tags: N-terminal His Tag
Subcellular Location: Mitochondrion inner membrane; Multi-pass membraneprotein.
Purity: >92%
Traits: Freeze-dried powder
Buffer formulation: 20mM Tris, 150mM NaCl, pH8.0, containing 1mMEDTA, 1mM DTT, 0.01% sarcosyl, 5%Trehalose and Proclin300.
Original Concentration: 200ug/mL
Applications: SDS-PAGE; WB; ELISA; IP; CoIP; Purification; Amine ReactiveLabeling.
(May be suitable for use in other assays to be determined by the end user.)
Predicted isoelectric point: 9.0
Predicted Molecular Mass: 14.0kDa
Accurate Molecular Mass: 14kDa as determined by SDS-PAGE reducingconditions.
[ USAGE ]
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0mg/mL. Do not vortex.
[ STORAGE AND STABILITY ]
Storage:Avoid repeated freeze/thaw cycles.
Store at 2-8°C for one month.
Aliquot and store at -80°C for 12 months.
Stability Test: The thermal stability is described by the loss rate. Thelossratewas determined by accelerated thermal degradation test, that is, incubatetheprotein at 37°C for 48h, and no obvious degradation and precipitationwereobserved. The loss rate is less than 5% within the expiration dateunderappropriate storage condition.
[ SEQUENCE ]
[ IDENTIFICATION ]
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