Anti-CD62E antibody
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概述
- 产品描述CD62E, known as E-selectin, is a cell adhesion molecule expressed only on endothelial cells activated by cytokines. Like other selectins, it plays an important part in inflammation. During inflammation, E-selectin plays an important part in recruiting leukocytes to the site of injury. The main ligands recognised by CD62E are oligosaccharides related to sialyl lewis x. P-selectin has a similar function, but is expressed on the endothelial cell surface within minutes as it is stored within the cell rather than produced on demand.
- 产品名称Anti-CD62E antibody
- 分子量67kDa
- 种属反应性Human
- 验证应用WB,ICC,FC
- 抗体类型兔多抗
- 免疫原This antibody is produced by immunizing rabbits with a synthetic peptide (KLH-coupled) corresponding to a region of N-terminal residues of CD62E.
- 偶联Non-conjugated
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性能
- 形态Liquid
- 浓度1 mg/mL.
- 存放说明Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
- 存储缓冲液1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
- 亚型IgG
- 纯化方式Peptide affinity purified.
- 亚细胞定位Cell membrane. Membrane.
- 数据链接P16581 Human
- 其它名称CD26E antibody
CD62 antigen-like family member E antibody
CD62E antibody
E selectin antibody
E-selectin antibody
ELAM antibody
ELAM-1 antibody
ELAM1 antibody
Endothelial adhesion molecule 1 antibody
Endothelial leukocyte adhesion molecule 1 antibody
ESEL antibody
LECAM2 antibody
Leukocyte-endothelial cell adhesion molecule 2 antibody
LYAM2_HUMAN antibody
Sele antibody
Selectin E antibody
more
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应用
WB: 1:500
ICC: 1:200
FC: 1:50-1:100
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Fig1: Western blot analysis of CD62E on different cell lysates using anti-CD62E antibody at 1/500 dilution.
Positive control:
Lane 1: MCF-7
Lane 2: Raji
Fig2: ICC staining CD62E in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig3: ICC staining CD62E in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig4: ICC staining CD62E in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig5: Flow cytometric analysis of Jurkat cells with CD62E antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
特别提示：本公司的所有产品仅可用于科研实验，严禁用于临床医疗及其他非科研用途！
