Anti-Pannexin 2 antibody

Anti-Pannexin 2 antibody

• 概述

• 产品描述The protein encoded by this gene belongs to the innexin family. Innexin family members are the structural components of gap junctions. This protein and pannexin 1 are abundantly expressed in central nervous system (CNS) and are coexpressed in various neuronal populations. Studies in Xenopus oocytes suggest that this protein alone and in combination with pannexin 1 may form cell type-specific gap junctions with distinct properties. Multiple transcript variants encoding different isoforms have been found for this gene. Structural component of the gap junctions and the hemichannels. Pannexin 2 is an important regulator of the insulin secretory capacity and apoptosis in pancreatic β-cells.
• 产品名称Anti-Pannexin 2 antibody
• 分子量74 kDa
• 种属反应性Human,Mouse,Rat
• 验证应用WB,IHC-P,ICC
• 抗体类型兔多抗
• 免疫原Synthetic peptide within Human Pannexin 2 71-95.
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度1 mg/mL.
• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
• 存储缓冲液1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
• 亚型IgG
• 纯化方式Peptide affinity purified.
• 亚细胞定位Cell membrane. Gap junction.
• 其它名称
• hPANX2 antibody
  Pannexin 2 antibody
  Pannexin-2 antibody
  PANX 2 antibody
  Panx2 antibody
  PANX2_HUMAN antibody
  Px2 antibody
  more

• 应用

  WB:1:500-1:2,000
  ICC:1:50-1:200
  IHC-P:1:100-1:400

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  Fig1: Western blot analysis of Pannexin 2 on HepG2 cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.

   

  

  Fig2: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-PANX2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibody for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chrogen. Counter stained with hematoxylin and mounted with DPX.

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