Anti-Kir6.2 antibody
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概述
- 产品描述Potassium channels are present in most mammalian cells, where they participate in a wide range of physiologic responses. The protein encoded by this gene is an integral membrane protein and inward-rectifier type potassium channel. The encoded protein, which has a greater tendency to allow Potassium to flow into a cell rather than out of a cell, is controlled by G-proteins and is found associated with the sulfonylurea receptor SUR. Mutations in this gene are a cause of familial persistent hyperinsulinemic hypoglycemia of infancy (PHHI), an autosomal recessive Disfect characterized by unregulated insulin secretion. Defects in this gene may also contribute to autosomal dominant non-insulin-dependent diabetes mellitus type II (NIDDM), transient neonatal diabetes mellitus type 3 (TNDM3), and permanent neonatal diabetes mellitus (PNDM). Multiple Alternative spliced transcript variants that encode different protein isoforms have been described for this gene.
- 产品名称Anti-Kir6.2 antibody
- 分子量44 kDa
- 种属反应性Human,Mouse,Rat
- 验证应用WB,IHC-P,ICC
- 抗体类型兔多抗
- 免疫原Recombinant protein within Human Kir6.2 aa 184-323.
- 偶联Non-conjugated
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性能
- 形态Liquid
- 浓度1 mg/mL.
- 存放说明Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
- 存储缓冲液1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
- 亚型IgG
- 纯化方式Protein affinity purified.
- 亚细胞定位Membrane.
- 其它名称ATP sensitive inward rectifier potassium channel 11 antibody
Beta cell inward rectifier subunit antibody
BIR antibody
HHF 2 antibody
HHF2 antibody
IKATP antibody
Inward rectifier K(+) channel Kir6.2 antibody
Inwardly rectifying potassium channel KIR6.2 antibody
IRK 11 antibody
IRK11 antibody
KCNJ11 antibody
Kir 6.2 antibody
Kir6.2 antibody
MGC133230 antibody
PHHI antibody
Potassium channel inwardly rectifing subfamily J member 11 antibody
Potassium channel, inwardly rectifying subfamily J member 11 antibody
Potassium inwardly rectifying channel J11 antibody
TNDM 3 antibody
TNDM3 antibody
more
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应用
WB:1:500-1:1,000
ICC:1:50-1:200
IHC-P:1:50-1:200
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Fig1: Western blot analysis of Kir6.2 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used at a 1:500 dilution in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Positive control:
Lane 1: Human liver tissue lysate
Lane 2: Mouse liver tissue lysate
Fig2: ICC staining of Kir6.2 in LoVo cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the antibodyat a dilution of 1:200 for 1 hour at room temperature, washed with PBS. Alexa Fluor®488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/100 dilution. The nuclear counter stain is DAPI (blue).
Fig3: Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue using anti-Kir6.2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibody at 1/200 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. Counter stained with hematoxylin and mounted with DPX.
Fig4: Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue using anti-Kir6.2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibody at 1/50 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. Counter stained with hematoxylin and mounted with DPX.
Fig5: Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-Kir6.2 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes.The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with ddH2O and PBS, and then probed with the antibody at 1/50 dilution, for 30 minutes at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. Counter stained with hematoxylin and mounted with DPX.
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