Anti-SYNPR/Synaptoporin antibody
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概述
- 产品描述Intrinsic membrane protein of small synaptic vesicles. Probable vesicular channel protein.
- 产品名称Anti-SYNPR/Synaptoporin antibody
- 分子量29 KDa
- 种属反应性Human,Mouse,Rat
- 验证应用WB,IHC-P,FC
- 抗体类型兔多抗
- 免疫原KLH conjugated synthetic peptide derived from human Synaptoporin 31-130/265
- 偶联Non-conjugated
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性能
- 形态Liquid
- 浓度1 mg/mL.
- 存放说明Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.
- 存储缓冲液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
- 亚型IgG
- 纯化方式affinity purified by Protein A
- 亚细胞定位Cytoplasmic vesicle > secretory vesicle > synaptic vesicle membrane. Cell junction > synapse > synaptosome.
- 其它名称SPO
Synaptophysin 2
Synaptoporin
Synpr
SYNPR_HUMAN
DKFZp686G0883
MGC26651.
more
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应用
WB:1:500-2000
IHC-P:1:400-800
FC:1μg/Test
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Fig1: Paraformaldehyde-fixed, paraffin embedded (rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (SYNPR) Polyclonal Antibody, Unconjugated at 1:800 overnight at 4℃, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.
Fig2: Blank control(blue): RSC96(fixed with 2% paraformaldehyde (10 min)).
Primary Antibody:Rabbit Anti-SYNPR/Synaptoporin antibodyDilution: 5μg in 100 μL 1X PBS containing 0.5% BSA;
Isotype Control Antibody: Rabbit IgG(orange) ,used under the same conditions );
Secondary Antibody: Goat anti-rabbit IgG-PE(white blue), Dilution: 1:200 in 1 X PBS containing 0.5% BSA.
Fig3: Sample:
Bulbus olfactorius (Rat) Lysate at 40 ug
Primary: Anti-Synaptoporin at 1/1000 dilution
Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
Predicted band size: 29 kD
Observed band size: 28 kD
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