Anti-GOLPH3 antibody
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概述
- 产品描述GOLPH3 (golgi phosphoprotein 3), also known as GOPP1, GPP34 or MIDAS, is a 298 amino acid protein that localizes to both the cytoplasm and the Golgi stack membrane where it is thought to play a regulatory role in protein trafficking within the Golgi. GOLPH3 is subject to post-translational phosphorylation and is encoded by a gene which maps to human chromosome 5. Chromosome 5 contains 181 million base pairs and comprises nearly 6% of the human genome. Chromosome 5 is associated with Cockayne syndrome through the ERCC8 gene and familial adenomatous polyposis through the adenomatous polyposis coli (APC) tumor suppressor gene. Treacher Collins syndrome is also chromosome 5-associated and is caused by insertions or deletions within the TCOF1 gene. Deletion of the p arm of chromosome 5 leads to Cri du chat syndrome, while deletion of the q arm or of chromosome 5 altogether is common in therapy-related acute myelogenous leukemias and myelodysplastic syndrome.
- 产品名称Anti-GOLPH3 antibody
- 分子量34 kDa
- 种属反应性Human,Mouse,Rat
- 验证应用WB,ICC,IHC-P,FC
- 抗体类型兔多抗
- 免疫原Recombinant protein
- 偶联Non-conjugated
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性能
- 形态Liquid
- 浓度1 mg/mL.
- 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
- 存储缓冲液1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
- 亚型IgG
- 纯化方式Protein A purified.
- 亚细胞定位Cell membrane. Endosome. Cytoplasm.
- 其它名称Coat protein antibody
Coat protein GPP34 antibody
FLJ90675 antibody
Golgi associated protein antibody
Golgi peripheral membrane protein 1, 34 kDa antibody
Golgi phosphoprotein 3 (coat protein) antibody
Golgi phosphoprotein 3 antibody
Golgi protein antibody
GOLP3_HUMAN antibody
Golph3 antibody
GOPP1 antibody
GPP34 antibody
MIDAS antibody
Mitochondrial DNA absence factor antibody
more
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应用
ICC: 1:50-1:200
IHC-P: 1:50-1:200
FC: 1:50-1:100
WB: 1:500
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Fig1: ICC staining GOLPH3 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig2: ICC staining GOLPH3 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig3: ICC staining GOLPH3 in SK-Br-3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig4: Immunohistochemical analysis of paraffin-embedded rat epididymis tissue using anti-GOLPH3 antibody. Counter stained with hematoxylin.
Fig5: Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue using anti-GOLPH3 antibody. Counter stained with hematoxylin.
Fig6: Immunohistochemical analysis of paraffin-embedded mouse stomach tissue using anti-GOLPH3 antibody. Counter stained with hematoxylin.
Fig7: Flow cytometric analysis of Hela cells with GOLPH3 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).
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