Anti-GAPDH antibody
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概述
- 产品描述Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC. Modulates the organization and assembly of the cytoskeleton. Facilitates the CHP1-dependent microtubule and membrane associations through its ability to stimulate the binding of CHP1 to microtubules (By similarity). Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate. Component of the GAIT (gamma interferon-activated inhibitor of translation) complex which mediates interferon-gamma-induced transcript-selective translation inhibition in inflammation processes.
- 产品名称Anti-GAPDH antibody
- 分子量36 kDa
- 种属反应性Human, Mouse, Rat
- 验证应用WB,ICC,IHC-P,FC
- 抗体类型兔多抗
- 免疫原Peptide
- 偶联Non-conjugated
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性能
- 形态Liquid
- 浓度1 mg/mL.
- 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
- 存储缓冲液1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
- 亚型IgG
- 纯化方式Peptide affinity purified
- 亚细胞定位Cytoskeleton. Cytosol. Nucleus.
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应用
WB: 1:2,000-5,000
ICC: 1:50-1:200
IHC-P: 1:50-1:200
FC: 1:50-1;100
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Fig1: Western blot analysis of GAPDH on different lysates using anti-GAPDH antibody at 1/2,000 dilution.
Positive control:
Lane 1: PC-12
Lane 2: L929
Lane 3: NIH/3T3
Lane 4: F9
Lane 5: Mouse liver tissue
Fig2: ICC staining GAPDH in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig3: Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-GAPDH antibody. Counter stained with hematoxylin.
Fig4: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-GAPDH antibody. Counter stained with hematoxylin.
Fig5: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-GAPDH antibody. Counter stained with hematoxylin.
Fig6: Flow cytometric analysis of MCF-7 cells with GAPDH antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.
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