Anti-RAB7 antibody
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概述
- 产品描述The Ras-related superfamily of guanine nucleotide binding proteins, which includes the Ral/Rec, Rap, R-Ras, and Rho/Rab subfamilies, exhibit 30-60% homology with Ras p21. Accumulating data suggests an important role for Rab proteins, either in endocytosis or in biosynthetic protein transport. The transport of newly synthesized proteins from the endoplasmic reticulum to various stacks of the Golgi complex and to secretory vesicles involves at each stage the movement of carrier vesicles, a process that appears to involve Rab protein function. The possibility that Rab proteins might also direct the exocytosis from secretory vesicles to the plasma membrane is supported by the observation that in yeast, the Sec4 protein, which is 40% homologous to Rab proteins, is associated with secretory vesicles. Several members of the Rab subfamily have been identified, each of which is found at a particular stage of a membrane transport pathway.
- 产品名称Anti-RAB7 antibody
- 分子量23 kDa
- 种属反应性Human,Mouse,Rat
- 验证应用WB,ICC,IHC-P,FC
- 抗体类型兔多抗
- 免疫原Full length recombinant protein of human RAB7.
- 偶联Non-conjugated
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性能
- 形态Liquid
- 浓度1 mg/mL.
- 存放说明Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
- 存储缓冲液1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
- 亚型IgG
- 纯化方式Protein affinity purified.
- 亚细胞定位Endosome. Lysosome.
- 其它名称CMT2B antibody
PRO2706 antibody
PSN antibody
RAB7, member RAS oncogene family antibody
RAB7A antibody
RAB7A, member RAS oncogene family antibody
RAB7A_HUMAN antibody
Ras associated protein RAB7 antibody
Ras related protein Rab7 antibody
Ras related protein Rab7a antibody
Ras-related protein Rab-7a antibody
more
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应用
WB: 1:500
ICC: 1:50-1:200
IHC-P: 1:50-1:200
FC: 1:50-1:100
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Fig1: Western blot analysis of RAB7 on MCF-7 cell lysate using anti-RAB7 antibody at 1/500 dilution.
Fig2: ICC staining RAB7 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig3: ICC staining RAB7 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig4: ICC staining RAB7 in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig5: Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-RAB7 antibody. Counter stained with hematoxylin.
Fig6: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-RAB7 antibody. Counter stained with hematoxylin.
Fig7: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-RAB7 antibody. Counter stained with hematoxylin.
Fig8: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-RAB7 antibody. Counter stained with hematoxylin.
Fig9: Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-RAB7 antibody. Counter stained with hematoxylin.
Fig10: Flow cytometric analysis of A431 cells with RAB7 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary
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