Anti-Cathepsin L antibody

Anti-Cathepsin L antibody

• 概述

• 产品描述Cathepsin L1 is a protein that in humans is encoded by the CTSL1 gene. The protein encoded by this gene is a lysosomal cysteine protease that plays a major role in intracellular protein catabolism. Its substrates include collagen and elastin, as well as alpha-1 protease inhibitor, a major controlling element of neutrophil elastase activity. The encoded protein has been implicated in several pathologic processes, including myofibril necrosis in myopathies and in myocardial ischemia, and in the renal tubular response to proteinuria. This protein, which is a member of the peptidase C1 family, is a dimer composed of disulfide-linked heavy and light chains, both produced from a single protein precursor. At least two transcript variants encoding the same protein have been found for this gene.

• 产品名称Anti-Cathepsin L antibody

• 分子量19/30/37 KDa

• 种属反应性Human,Mouse,Rat

• 验证应用WB,IHC-P

• 抗体类型兔多抗

• 免疫原KLH conjugated synthetic peptide derived from human cathepsin L1 proprotein 71-170/334

• 偶联Non-conjugated

• 性能

• 形态Liquid

• 浓度1 mg/mL.

• 存放说明Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.

• 存储缓冲液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

• 亚型IgG

• 纯化方式affinity purified by Protein A

• 亚细胞定位Lysosome.

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• ​其它名称Cathepsin L heavy chain
  Cathepsin L light chain
  CathepsinL
  CATL
  CTSL
  CTSL protein
  DKFZp686A18159
  FLJ31037
  Major excreted protein
  MEP
  cath-L
  CL
  CATL1_HUMAN
  CathepsinL1
  Cathepsin L1,Cathepsin-L1
  CTSL 1
  CTSL-1,CTS L1,CTS-L1.

  more

• 应用

  WB:1:500-2000
  IHC-P:1:400-800

•  

  

  Fig1: Sample:
  A549 Cell (Human) Lysate at 30 ug
  K562 Cell (Human) Lysate at 30 ug
  Primary: Anti- Cathepsin L  at 1/300 dilution
  Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
  Predicted band size: 19/30/37 kD
  Observed band size: 40/63 kD

   

  

  Fig2: Protein: line1, rat brain lysates, 30ug; line2, rat liver lysates, 30ug;
  Primary: Anti-Cathepsin L  at 1:200;
  Secondary: HRP conjugated Goat-Anti-Rabbit IgG(bs-0295G-HRP) at 1: 3000;
  ECL excitated the fluorescence;
  Predicted band size : 37kD
  Observed band size : 19kD, 37kD

   

  

  Fig3: Tissue/cell: human cervical carcinoma; 4% Paraformaldehyde-fixed and paraffin-embedded;
  Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
  Incubation: Anti-Cathepsin Polyclonal Antibody, Unconjugated 1:500, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining

   

  

  Fig4: Sample:
  D5-16 Cell (Human) Lysate at 30 ug
  D5-23 Cell (Human) Lysate at 40 ug
  Primary: Anti- Cathepsin L  at 1/300 dilution
  Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
  Predicted band size: 19/30/37 kD
  Observed band size: 40/63 kD

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