Anti-Glucagon Receptor antibody

Anti-Glucagon Receptor antibody

• 概述

• 产品描述This is a receptor for glucagon which plays a central role in regulating the level of blood glucose by controlling the rate of hepatic glucose production and insulin secretion. The activity of this receptor is mediated by G proteins which activate adenylyl cyclase and also a phosphatidylinositol-calcium second messenger system.

• 产品名称Anti-Glucagon Receptor antibody

• 分子量51 KDa

• 种属反应性Human,Mouse,Rat,Chicken,Dog,Pig,Cow,Guinea Pig

• 验证应用WB,IHC-P,FC

• 抗体类型兔多抗

• 免疫原KLH conjugated synthetic peptide derived from human Glucagon Receptor 11-110/477

• 偶联Non-conjugated

• 性能

• 形态Liquid

• 浓度1 mg/mL.

• 存放说明Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.

• 存储缓冲液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

• 亚型IgG

• 纯化方式affinity purified by Protein A

• 亚细胞定位Cell membrane; Multi-pass membrane protein.

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• ​其它名称GCGR
  GGR
  GL R
  GL-R
  GLR
  GLR_HUMAN
  MGC138246
  Glucagon receptor precursor.

  more

• 应用

  WB:1:500-2000
  IHC-P:1:400-800
  FC:1μg/Test

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  Fig1: Paraformaldehyde-fixed, paraffin embedded (rat brain tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (Glucagon Receptor) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

   

  

  Fig2: Paraformaldehyde-fixed, paraffin embedded (human stomach tissue); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (Glucagon Receptor) Polyclonal Antibody, Unconjugated at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

   

  

  Fig3: Blank control(blue): Molt-4 Cells(fixed with 2% paraformaldehyde (10 min)).
  Primary Antibody: Rabbit Anti-Glucagon Receptor/AF647 Conjugated antibody , Dilution: 1μg in 100 μL 1X PBS containing 0.5% BSA;
  Isotype Control Antibody: Rabbit IgG/AF647(orange) ,used under the same conditions.

   

  

  Fig4: Sample: Cerebellum (Mouse) Lysate at 40 ug
  Primary: Anti-Glucagon Receptor  at 1/300 dilution
  Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
  Predicted band size: 51 kD
  Observed band size: 51 kD

   

  

  Fig5: Sample:Kidney (Mouse) Lysate at 40 ug
  Primary: Anti-Glucagon Receptor)at 1/300 dilution
  Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
  Predicted band size: 51kD
  Observed band size: 51kD

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