Anti-HRG beta 1 antibody

Anti-HRG beta 1 antibody

• 概述

• 产品描述Direct ligand for ERBB3 and ERBB4 tyrosine kinase receptors. Concomitantly recruits ERBB1 and ERBB2 coreceptors, resulting in ligand-stimulated tyrosine phosphorylation and activation of the ERBB receptors. The multiple isoforms perform diverse functions such as inducing growth and differentiation of epithelial, glial, neuronal, and skeletal muscle cells; inducing expression of acetylcholine receptor in synaptic vesicles during the formation of the neuromuscular junction; stimulating lobuloalveolar budding and milk production in the mammary gland and inducing differentiation of mammary tumor cells; stimulating Schwann cell proliferation; implication in the development of the myocardium such as trabeculation of the developing heart. Isoform 10 may play a role in motor and sensory neuron development.

• 产品名称Anti-HRG beta 1 antibody

• 分子量70 KDa

• 种属反应性Human,Mouse,Rat,Rabbit

• 验证应用WB,IHC-P,ICC/IF

• 抗体类型兔多抗

• 免疫原KLH conjugated synthetic peptide derived from human Neuregulin 1 65-150/640

• 偶联Non-conjugated

• 性能

• 形态Liquid

• 浓度1 mg/mL.

• 存放说明Store at -20℃ for one year. Avoid repeated freeze/thaw cycles.

• 存储缓冲液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

• 亚型IgG

• 纯化方式Affinity purified by Protein A

• 亚细胞定位Pro-neuregulin-1, membrane-bound isoform: Cell membrane; Single-pass type I membrane protein. Neuregulin-1: Secreted. Isoform 8: Nucleus. Isoform 9: Secreted. Isoform 10: Membrane; Single-pass type I membrane protein.

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• ​其它名称Neuregulin 1
  GGF2
  Acetylcholine receptor inducing activity
  ARIA
  Breast cancer cell differentiation factor p45
  GGF
  glial growth factor
  Heregulin
  Heregulin beta1
  heregulin, alpha (45kD, ERBB2 p185 activator)
  HGL
  HRG
  HRG beta 1A
  HRG1
  HRGA
  NDF
  neu differentiation factor
  NRG1
  sensory and motor neuron derived factor
  SMDF
  NRG1_HUMAN.

  more

• 应用

  WB:1:500-2000
  IHC-P:1:400-800
  IF:1:200-800
  ICC/IF:1:100-500

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  Fig1: Sample:
  Brain (Mouse) Lysate at 40 ug
  Raji Cell (Human) Lysate at 40 ug
  Primary: Anti-HRG beta 1  at 1/300 dilution
  Secondary: HRP conjugated Goat-Anti-rabbit IgG (bs-0295G-HRP) at 1/5000 dilution
  Predicted band size: 70 kD
  Observed band size: 65 kD

   

  

  Fig2: Tissue/cell: Mouse ovary tissue; 4% Paraformaldehyde-fixed and paraffin-embedded;
  Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37℃ for 20 min;
  Incubation: Anti-HRG beta 1 Polyclonal Antibody, Unconjugated( 1:200, overnight at 4℃, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining

   

  

  Fig3: Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (HRG beta 1) Polyclonal Antibody, Unconjugated  at 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

   

  

  Fig4: Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (HRG beta 1) Polyclonal Antibody, Unconjugatedat 1:400 overnight at 4℃, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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