Anti-REC8 antibody

Anti-REC8 antibody

• 概述

• 产品描述Required during meiosis for separation of sister chromatids and homologous chromosomes. Proteolytic cleavage of REC8 on chromosome arms by separin during anaphase I allows for homologous chromosome separation in meiosis I and cleavage of REC8 on centromeres during anaphase II allows for sister chromatid separation in meiosis II (By similarity).

• 产品名称Anti-REC8 antibody

• 分子量60 KDa

• 种属反应性Human,Mouse,Rat,Dog,Pig,Horse,Rabbit,Guinea Pig

• 验证应用WB,FC

• 抗体类型兔多抗

• 免疫原KLH conjugated synthetic peptide derived from human REC8 22-120/547

• 偶联Non-conjugated

• 性能

• 形态Liquid

• 浓度1 mg/mL.

• 存放说明Store at -20℃ for one year. Avoid repeated freeze/thaw cycles. The lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. When reconstituted in sterile pH 7.4 0.01M PBS or diluent of antibody the antibody is stable for at least two weeks at 2-4℃.

• 存储缓冲液0.01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.

• 亚型IgG

• 纯化方式affinity purified by Protein A

• 亚细胞定位Nucleus. Chromosome.

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• 其它名称Cohesin Rec8p
  Meiotic recombination protein REC8 homolog
  Meiotic recombination protein REC8-like 1
  rec8
  REC8 homolog (yeast)
  REC8_HUMAN
  REC8L
  Rec8L1
  Rec8p.

  more

• 应用

  WB:1:500-2000
  FC:1ug/Test

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  Fig1: Blank control (Black line):Molt4 (Black).
  Primary Antibody (green line): Rabbit Anti-REC8 antibody 
  Dilution: 1μg /10^6 cells;
  Isotype Control Antibody (orange line): Rabbit IgG .
  Secondary Antibody (white blue line): Goat anti-rabbit IgG-AF647
  Dilution: 1μg /test.
  Protocol
  The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 90% ice-cold methanol for 20 min at room temperature. The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.

   

  

  Fig2: Sample: HT29 Cell (Human) Lysate at 40 ug
  Primary: Anti-REC8  at 1/300 dilution
  Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
  Predicted band size: 60 kD
  Observed band size: 65 kD

   

  

  Fig3: Sample:
  Thymus (Mouse) Lysate at 40 ug
  Primary: Anti-REC8 ( at 1/1000 dilution
  Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution
  Predicted band size: 60 kD
  Observed band size: 60 kD

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特别提示：本公司的所有产品仅可用于科研实验，严禁用于临床医疗及其他非科研用途！