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Anti-PDI antibody
产品描述Protein disulfide isomerase or PDI is an enzyme in the endoplasmic reticulum in eukaryotes that catalyzes the formation and breakage of disulfide bonds between cysteine residues within proteins as they fold. Another major function of PDI relates to its activity as a chaperone; i.e., it aids wrongly folded proteins to reach a correctly folded state without the aid of enzymatic disulfide shuffling. PDI has been found to be involved in the breaking of bonds on the HIV gp120 protein during HIV infection of CD4 positive cells, and is required for HIV infection of lymphocytes and monocytes.
产品名称Anti-PDI antibody
分子量58 kDa
种属反应性Human,Mouse,Rat
验证应用WB,ICC,IHC-P
抗体类型兔多抗
免疫原peptide
偶联Non-conjugated
形态Liquid
浓度1 mg/mL.
存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
存储缓冲液1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
亚型IgG
纯化方式Peptide affinity purified
亚细胞定位Endoplasmic reticulum lumen
其它名称
WB: 1:500-1:1,000
ICC: 1:200
IHC-P: 1:200
Fig1: Western blot analysis of PDI on different cell lysates using anti- PDI antibody at 1/1000 dilution.
Positive control:
Lane 1: Hela Lane 2: MCF-7
Lane 3: PANC Lane 4: HepG2
Lane 5: HUVEC Lane 6: NIH/3T3
Lane 7: L929 Lane 8: F9
Lane 9: Jurkat Lane 10: A431
Lane 11: A549 Lane 12: Mouse liver
Lane 13: Mouse kidney Lane 14: Mouse brain
Lane 15: Human kidney Lane 16: Human brain
Lane 17: Human liver
Fig2: ICC staining PDI in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig3: ICC staining PDI in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig4: ICC staining PDI in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig5: Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using anti-PDI antibody. Counter stained with hematoxylin.
Fig6: Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using anti-PDI antibody. Counter stained with hematoxylin.
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