Anti-GRP78 antibody

Anti-GRP78 antibody

• 概述

• 产品描述Binding immunoglobulin protein (BiP) also known as 78 kDa glucose-regulated protein (GRP-78) or heat shock 70 kDa protein 5 (HSPA5) is a protein that in humans is encoded by the HSPA5 gene. BiP is a HSP70 molecular chaperone located in the lumen of the endoplasmic reticulum (ER) that binds newly synthesized proteins as they are translocated into the ER, and maintains them in a state competent for subsequent folding and oligomerization. BiP is also an essential component of the translocation machinery, as well as playing a role in retrograde transport across the ER membrane of aberrant proteins destined for degradation by the proteasome. Like many stress and heat shock proteins, BiP/GRP78 has potent immunological activity when released from the internal environment of the cell into the extracelluar space.specifically, it feeds anti-inflammatory and pro-resolutory signals into immune networks, thus helping to resolve inflammation.

• 产品名称Anti-GRP78 antibody

• 分子量72 kDa

• 种属反应性Human,Mouse,Rat

• 验证应用WB,ICC,IHC-P,FC

• 抗体类型兔多抗

• 免疫原peptide

• 偶联Non-conjugated

• 性能

• 形态Liquid

• 浓度1 mg/mL.

• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.

• 存储缓冲液1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

• 亚型IgG

• 纯化方式Peptide affinity purified

• 亚细胞定位Cytoplasm, endoplasmic reticulum lumen

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• 其它名称78 kDa glucose regulated protein antibody
  78 kDa glucose-regulated protein antibody
  AL022860 antibody
  AU019543 antibody
  BIP antibody
  D2Wsu141e antibody
  D2Wsu17e antibody
  Endoplasmic reticulum lumenal Ca(2+)-binding protein grp78 antibody
  Endoplasmic reticulum lumenal Ca2+ binding protein grp78 antibody
  Epididymis secretory sperm binding protein Li 89n antibody
  FLJ26106 antibody
  Glucose Regulated Protein 78kDa antibody
  GRP 78 antibody
  GRP-78 antibody
  GRP78 antibody
  GRP78_HUMAN antibody
  Heat shock 70 kDa protein 5 antibody
  Heat Shock 70kDa Protein 5 antibody
  Heat shock protein family A (Hsp70) member 5 antibody
  HEL S 89n antibody
  Hsce70 antibody
  HSPA 5 antibody
  HSPA5 antibody
  Immunoglobulin Heavy Chain Binding Protein antibody
  Immunoglobulin heavy chain-binding protein antibody
  mBiP antibody
  MIF2 antibody
  Sez7 antibody

  more

• 应用

  WB: 1:2,000
  ICC: 1:200
  IHC-P: 1:200
  FC: 1:100-1:200

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  Fig1: Western blot analysis of GRP78 on different cell lysates using anti-GRP78 antibody at 1/2000 dilution.

   

  

  Fig2: ICC staining GRP78 in Hela cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

   

  

  Fig3: ICC staining GRP78 in SK-BR-3 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

   

  

  Fig4: ICC staining GRP78 in HepG2 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

   

  

  Fig5: IF staining GRP78 in Hela cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS and counterstained with DAPI in order to highlight the nucleus (blue).

   

  

  Fig6: Immunohistochemical analysis of paraffin-embedded rat small intestine tissue using anti-GRP78 antibody. Counter stained with hematoxylin.

   

  

  Fig7: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-GRP78 antibody. Counter stained with hematoxylin.

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