Anti-Nox3 antibody
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概述
- 产品描述This gene encodes a member of the NOX family of NADPH oxidases. These enzymes have the capacity to generate superoxide and other reactive oxygen species (ROS) and transport electrons across the plasma membrane. The ROS generated by family members have been implicated in numerous biological functions including host defense, posttranlational processing of proteins, cellular signaling, regulation of gene expression, and cell differentiation. The protein encoded by this gene is expressed predominantly in the inner ear and is involved in the biogenesis of otoconia/otolith, which are crystalline structures of the inner ear involved in the perception of gravity.,
- 产品名称Anti-Nox3 antibody
- 分子量65 kDa
- 种属反应性Human,Mouse,Rat
- 验证应用WB,IHC-P
- 抗体类型兔多抗
- 免疫原The antiserum was produced against synthesized peptide derived from human NOX3. AA range:287-336
- 偶联Non-conjugated
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性能
- 形态Liquid
- 浓度1 mg/mL.
- 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
- 存储缓冲液Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.
- 亚型IgG
- 纯化方式Immunogen affinity purified.
- 亚细胞定位Membrane
- 其它名称
- NOX3 antibody; MOX2 antibody; NADPH oxidase 3 antibody; Mitogenic oxidase 2 antibody; MOX-2 antibody; gp91phox homolog 3 antibody; GP91-3 antibody;
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应用
WB: 1:500-1:2000
IHC-P: 1:100-1:300
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Fig1: Western blot analysis of Nox3 on A543 lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER64216, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
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