Anti-IRS-2 antibody
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概述
- 产品描述IRS-2, originally described as 4PS, acts as a signaling intermediate downstream of the Insulin, IGF-1, IL-4, IL-9 and IL-13 receptors. In IRS-2-deficient mice, reduction in total PI 3-kinase activity by 30% and abolition of downstream activation of protein kinase C (PKC) ζ leads to the development of type 2 diabetes. Additionally, reconstitution with retroviral IRS-2 restores IRS-2/PI 3-kinase/PKC ζ signalling as well as glucose uptake. IRS-2 translocates to the nuclei of mouse embryo fibroblasts expressing the Insulin-like growth factor 1 receptor. Various mutations in the IGF-IR can result in an abrogation of or decrease in the translocation of IRS proteins to the nucleoli. IRS-2 is responsible for mitogen-activated protein kinase (MAPK) and protein kinase B (PKB) activation by Insulin and is the major adapter molecule linking the Insulin receptor to this step.
- 产品名称Anti-IRS-2 antibody
- 分子量165-185kDa
- 种属反应性Human,Mouse,Rat
- 验证应用WB,IP,IF
- 抗体类型兔多抗
- 免疫原Amino acids 926-1130 of IRS-2 of human origin.
- 偶联Non-conjugated
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性能
- 形态Liquid
- 浓度1 mg/mL.
- 存放说明Store at +4℃
- 存储缓冲液1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
- 亚型IgG
- 纯化方式Immunogen affinity purified
- 亚细胞定位Cytoplasm
- 其它名称
- Insulin receptor substrate 2 antibody
- IRS 2 antibody
- IRS-2 antibody
more
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应用
WB: 1:100-1:1,000
IP: 1-2 μg per 100-500 μg of total protein (1 ml of cell lysate)
IF: 1:50-1:500
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Fig1: Western blot analysis of IRS-2 expression in HeLa (A) and 3T3-L1 (B) whole cell lysates.
Fig2: Immunofluorescence staining of methanol-fixed 3T3-L1 cells showing cytoplasmic staining (A). Immunoperoxidase staining of formalin fixed, paraffin-embedded human skeletal muscle tissue showing cytoplasmic staining of myocytes (B).
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