Anti-SARS2 antibody
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概述
- 产品描述Involved in ATP binding, has serine-tRNA ligase activity and involved in seryl-tRNA aminoacylation.
- 产品名称Anti-SARS2 antibody
- 分子量58 kDa
- 种属反应性Zebrafish
- 验证应用WB,IF, IHC-P
- 抗体类型兔多抗
- 免疫原Recombinant protein within Zebrafish SARS2 aa 293-514.
- 偶联Non-conjugated
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性能
- 形态Liquid
- 浓度1 mg/mL.
- 存放说明Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
- 存储缓冲液1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
- 亚型IgG
- 纯化方式Protein affinity purified.
- 亚细胞定位Cytoplasm.
- 其它名称
- mitochondrial antibody
- mtSerRS antibody
- SARS antibody
more
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应用
WB: 1:500
IF: 1:50-1:200
IHC-P: 1:50-1:200
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Fig1: Western blot analysis of SARS2 on Zebrafish tissue lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (ER1902-38, 1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.
Fig2: IF staining of SARS2 in Zebrafish (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig3: Immunohistochemical analysis of paraffin-embedded Zebrafish tissue using anti-SARS2 antibody. Counter stained with hematoxylin.
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