Anti-Cytochrome C antibody
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概述
- 产品描述Cytochrome c is a well characterized mobile electron transport protein that is essential to energy conversion in all aerobic organisms. In mammalian cells, this highly conserved protein is normally localized to the mitochondrial intermembrane space. More recent studies have identifed cytosolic cytochrome c as a factor necessary for activation of apoptosis. During apoptosis, cytochrome c is translocated from the mitochondrial membrane to the cytosol, where it is required for activation of caspase-3 (CPP32). Overexpression of Bcl-2 has been shown to prevent the translocation of cytochrome c, thereby blocking the apoptotic process. Overexpression of Bax has been shown to induce the release of cytochrome c and to induce cell death. The release of cytochrome c from the mitochondria is thought to trigger an apoptotic cascade, whereby Apaf-1 binds to Apaf-3 (caspase-9) in a cytochrome c-dependent manner, leading to caspase-9 cleavage of caspase-3.
- 产品名称Anti-Cytochrome C antibody
- 分子量12kDa
- 种属反应性Human,Mouse,Rat
- 验证应用WB,ICC,IHC-P,FC
- 抗体类型兔多抗
- 免疫原peptide
- 偶联Non-conjugated
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性能
- 形态Liquid
- 浓度1 mg/mL.
- 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
- 存储缓冲液1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
- 亚型IgG
- 纯化方式Peptide affinity purified
- 亚细胞定位Mitochondrion intermembrane space
- 其它名称
- CYC antibody
- CYC_HUMAN antibody
- CYCS antibody
more
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应用
WB: 1:1,000-1:5,000
ICC: 1:50-1:200
IHC-P: 1:50-1:200
FC: 1:50-1:100
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Fig1: Western blot analysis on mouse brain(1) mouse heart(2) and mouse kidney(3) cell lysates using anti-Cytochrome C rabbit polyclonal antibody.
Fig2: Immunocytochemical staining of Hela cells using anti-Cytochrome C rabbit polyclonal antibody.
Fig3: Immunocytochemical staining of HepG2 cells using anti-Cytochrome C rabbit polyclonal antibody.
Fig4: Immunocytochemical staining of MCF-7 cells using anti-Cytochrome C rabbit polyclonal antibody.
Fig5: Immunocytochemical staining of 293 cells using anti-Cytochrome C rabbit polyclonal antibody.
Fig6: Immunohistochemical analysis of paraffin- embedded human spleen tissue using anti-Cytochrome C rabbit polyclonal antibody.
Fig7: Immunohistochemical analysis of paraffin- embedded human kidney tissue using anti-Cytochrome C rabbit polyclonal antibody.
Fig8: Immunohistochemical analysis of paraffin- embedded mouse liver tissue using anti-Cytochrome C rabbit polyclonal antibody.
Fig9: Immunohistochemical analysis of paraffin- embedded mouse spleen tissue using anti-Cytochrome C rabbit polyclonal antibody.
Fig10: Immunohistochemical analysis of paraffin- embedded mouse kidney tissue using anti-Cytochrome C rabbit polyclonal antibody.
Fig11: Immunohistochemical analysis of paraffin- embedded human liver tissue using anti-Cytochrome C rabbit polyclonal antibody.
Fig12: Immunohistochemical analysis of paraffin- embedded mouse heart tissue using anti-Cytochrome C rabbit polyclonal antibody.
Fig13: Flow cytometric analysis of Hela cells with Cytochrome C antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated Goat anti rabbit IgG was used as the sec
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