Anti-NF-κB p105/p50 antibody
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概述
- 产品描述NF-kappa-B is a pleiotropic transcription factor present in almost all cell types and is the endpoint of a series of signal transduction events that are initiated by a vast array of stimuli related to many biological processes such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52 and the heterodimeric p65-p50 complex appears to be most abundant one. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. NF-kappa-B is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by interactions with other cofactors or corepressors. NF-kappa-B heterodimeric p65-p50 and RelB-p50 complexes are transcriptional activators.
- 产品名称Anti-NF-κB p105/p50 antibody
- 分子量~50/105kDa
- 种属反应性Human,Mouse
- 验证应用WB,ICC,IHC-P
- 抗体类型兔多抗
- 免疫原This antibody is produced by immunizing rabbits with a synthetic peptide (KLH-coupled) corresponding to residues in the nuclear factor NF-κB p50 subunit.
- 偶联Non-conjugated
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性能
- 形态Liquid
- 浓度1 mg/mL.
- 存放说明Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
- 存储缓冲液1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
- 亚型IgG
- 纯化方式Immunogen affinity purified
- 亚细胞定位Nucleus. Cytoplasm.
- 其它名称
- DKFZp686C01211 antibody
- DNA binding factor KBF1 antibody
- DNA binding factor KBF1 EBP1 antibody
more
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应用
WB: 1:500-1:1,000
ICC: 1:200-1:500
IHC-P: 1:200
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Fig1: Western blot analysis on Hela (1), HepG2 (2) and Raji (3) cell lysates using anti-NF-κB p105/p50 rabbit polyclonal antibodies.
Fig2: Immunofluorescent staining of Hela cells using anti-NF-κB p105/p50 rabbit polyclonal antibody.
Fig3: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-NF-κB p105/p50 rabbit polyclonal antibody.
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