Anti-RPL18A antibody [G10-G6]

Anti-RPL18A antibody [G10-G6]

• 概述

• 产品描述Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a member of the L18AE family of ribosomal proteins that is a component of the 60S subunit. The encoded protein may play a role in viral replication by interacting with the hepatitis C virus internal ribosome entry site (IRES). This gene is co-transcribed with the U68 snoRNA, located within the third intron. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed throughout the genome.
• 产品名称Anti-RPL18A antibody [G10-G6]
• 分子量21 kDa
• 种属反应性Human,Mouse
• 验证应用WB,ICC,IHC-P,FC
• 抗体类型小鼠单抗
• 免疫原Recombinant protein
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度2 mg/mL.
• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
• 存储缓冲液1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
• 亚型IgG1
• 纯化方式Protein A purified.
• 亚细胞定位Cytosolic large ribosomal subunit.
• 其它名称
  • 2510019J09Rik antibody
  • 60S ribosomal protein L18a antibody
  • L18A antibody
  more

• 应用

  WB: 1:500-1:2,000
  ICC: 1:50-1:200
  IHC-P: 1:100-1:200
  FC: 1:100-1:200

•  

  

  Fig1: Western blot analysis of RPL18A on human RPL18A recombinant protein using anti-RPL18A antibody at 1/1,000 dilution.

   

  

  Fig2: Western blot analysis of RPL18A on different cell lysate using anti-RPL18A antibody at 1/1,000 dilution.
  Positive control:
  Line1:NIH3T3
  Line2:HEK293
  Line3:HL60
  Line4:Jurka
  Line5:Raji
  Line6:MOLT4
  Line7:HeLa

   

  

  Fig3: ICC staining RPL18A (green) in HepG2 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

   

  

  Fig4: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-RPL18A antibody. Counter stained with hematoxylin.

   

  

  Fig5: Flow cytometric analysis of HEK293 cells with RPL18A antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; purple).

特别提示：本公司的所有产品仅可用于科研实验，严禁用于临床医疗及其他非科研用途！