Anti-Alpha-tubulin antibody [A8-6]

Anti-Alpha-tubulin antibody [A8-6]

• 概述

• 产品描述The cytoskeleton consists of three types of cytosolic fibers: microtubules, microfilaments (actin filaments), and intermediate filaments. Globular tubulin subunits comprise the microtubule building block, with α/β-tubulin heterodimers forming the tubulin subunit common to all eukaryotic cells. Acetylation of alpha chains at Lys-40 stabilizes microtubules and affects affinity and processivity of microtubule motors. This modification has a role in multiple cellular functions, ranging from cell motility, cell cycle progression or cell differentiation to intracellular trafficking and signaling.
• 产品名称Anti-Alpha-tubulin antibody [A8-6]
• 分子量50 kDa
• 种属反应性Human,Mouse,Rat, Zebrafish
• 验证应用WB, ICC, IHC-P, FC
• 抗体类型小鼠单抗
• 免疫原peptide
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度2 mg/mL.
• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
• 存储缓冲液1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
• 亚型IgG3
• 纯化方式Protein A purified.
• 亚细胞定位Cytoplasm ,cytoskeleton
• 其它名称
  • TUBA1 antibody
  • TUBA1A antibody
  • TUBA2 antibody
  more

• 应用

  WB: 1:5,000
  ICC: 1:500

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  Fig1: Western blot analysis on PC12 cell lysates using anti-α-tubulin mouse mAb.

   

  

  Fig2: ICC staining α-tubulin in Hela cells (red). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

   

  

  Fig3: Western blot analysis of α-tubulin on hybrid fish (crucian-carp) brain tissue lysate using anti-α-tubulin antibody at 1/500 dilution.

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