Anti-Phospho-4E-BP1/2/3 (Thr 45) antibody [3G2]

Anti-Phospho-4E-BP1/2/3 (Thr 45) antibody [3G2]

• 概述

• 产品描述The multisubunit eukaryotic translation initiation factor (eIF) 4F recruits 40S ribosomal subunits to the 5' end of mRNA. The eIF4F subunit eIF4E interacts directly with the mRNA 5' cap structure. Assembly of the eIF4F complex is inhibited by a family of repressor polypeptides, the eIF4E-binding proteins (4E-BPs). 4E-BP1 (also known as PHAS-1) normally binds eIF4E, inhibiting cap-dependent translation. Hyper-phosphorylation of 4E-BP1 disrupts this binding, activating cap-dependent translation. The PI3-kinase/Akt pathway and the FRAP/mTOR kinase regulate 4E-BP1. 4E-BP1 is phosphorylated in vivo on multiple residues and phosphorylation by FRAP/mTOR on Threonine 37 and Threonine 46 of human 4E-BP1 may prime it for sub-sequent phosphorylation at sites including Serine 65 and Threonine 70. The corresponding rat residues include Threonine 36, Threonine 45, Serine 64 and Threonine 69. In vitro, 4E-BP1 is also phosphorylated by ataxia telangiectasia (ATM) at human Serine 112 (rat Serine 111) in response to an increase in insulin levels.
• 产品名称Anti-Phospho-4E-BP1/2/3 (Thr 45) antibody [3G2]
• 分子量21 kDa
• 种属反应性Human,Mouse,Rat
• 验证应用WB,IP,IF
• 抗体类型小鼠单抗
• 免疫原peptide
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度2 mg/mL.
• 存放说明Store at +4℃
• 存储缓冲液1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
• 亚型IgG2a
• 纯化方式Protein A purified.
• 亚细胞定位Cytoplasm
• 其它名称
  • 4E-BP1 antibody
  • 4EBP1 antibody
  • 4EBP1_HUMAN antibody
  more

• 应用

  WB: 1:100-1:1,000
  IP: 1-2 μg per 100-500 μg of total protein(1 ml of cell lysate)
  IF: 1:50-500

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  Fig1: Westernblot analysis of 4E-BP1/2/3 phosphorylation inuntreated (A), calyculinA treated (B) and calyculin A and lambda protein phosphatase treated (C) Jurkat whole cell lysates.

   

  

  Fig2: Western blot analysis of 4E-BP1 phosphorylation in nontransfected (A,D), untreated human 4E-BP1 transfected (B,E) and lambda protein phosphatase treated human 4E-BP1 transfected (C,F) 293T whole cell lysates. Antibodies tested include p-4E-BP1/2/3 (A,B,C) and 4E-BP1 (D,E,F).

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