Anti-Histone H3 antibody [6-A7]
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概述
- 产品描述The nucleosome, made up of DNA wound around eight core histone proteins (two each of H2A, H2B, H3, and H4), is the primary building block of chromatin. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
- 产品名称Anti-Histone H3 antibody [6-A7]
- 分子量17 kDa
- 种属反应性Human, Mouse, Rat
- 验证应用WB, ICC, IHC-P, FC
- 抗体类型小鼠单抗
- 免疫原Synthetic peptide.
- 偶联Non-conjugated
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性能
- 形态Liquid
- 浓度2 mg/mL.
- 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
- 存储缓冲液1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
- 亚型IgG1
- 纯化方式Protein A purified.
- 亚细胞定位Nucleus.
- 其它名称
- H3 histone family, member A antibody
- H3/A antibody
- H31_HUMAN antibody
more
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应用
WB: 1:10,000-1:20,000
ICC: 1:200
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Fig1: Western blot analysis on Hela (1), NIH/3T3 (2), PC12(3) cell lysates using anti- Histone H3 Mouse mAb (Cat. # M1306-4).
Fig2: ICC staining Histone H3 in Hela cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig3: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti- Histone H3 mouse monoclonal antibody.
Fig4: Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti- Histone H3 mouse monoclonal antibody.
Fig5: Immunofluorescence analysis of paraffin-embedded rat brain tissue using anti-Histone H3 mouse monoclonal antibody.
Fig6: Flow cytometric analysis of Hela cells with Histone H3 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; blue). Alexa Fluor 488-conjugated goat anti mouse IgG was used as the secondary antibody.
特别提示：本公司的所有产品仅可用于科研实验，严禁用于临床医疗及其他非科研用途！
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