Anti-MAP2K4 antibody [B4-H6]

Anti-MAP2K4 antibody [B4-H6]

• 概述

• 产品描述A family of protein kinases located upstream of the MAP kinases and responsible for their activation has been identified. The prototype member of this family, designated MAP kinase kinase, or MEK-1, specifically phosphorylates the MAP kinase regulatory threonine and tyrosine residues present in the Thr-Glu-Tyr motif of ERK. A second MEK family member, MEK-2, resembles MEK-1 in its substrate specificity. MEK-3 (or MKK-3) functions to activate p38 MAP kinase, and MEK-4 (also called SEK1 or MKK-4) activates both p38 and JNK MAP kinases. MEK-5 appears to specifically phosphorylate ERK5, whereas MEK-6 phosphorylates p38 and p38b. MEK-7 (or MKK-7) phosphorylates and activates the JNK signal transduction pathway.
• 产品名称Anti-MAP2K4 antibody [B4-H6]
• 分子量44 kDa
• 种属反应性Human
• 验证应用WB,IHC-P,FC
• 抗体类型小鼠单抗
• 免疫原Recombinant protein
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度2 mg/mL.
• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
• 存储缓冲液1*TBS (pH7.4), 1%BSA, Preservative: 0.05% Sodium Azide.
• 亚型IgG1
• 纯化方式Protein A purified.
• 亚细胞定位Cytoplasm. Nucleus.
• 其它名称
  • c Jun N terminal kinase kinase 1 antibody
  • C-JUN N-terminal kinase kinase 1 antibody
  • Dual specificity mitogen activated protein kinase kinase 4 antibody
  more

• 应用

  WB: 1:500-1:1,000
  IHC-P: 1:50-1:200
  FC: 1:100-1:200

•  

  

  Fig1: Western blot analysis of MAP2K4 on different cell lysates using anti-MAP2K4 antibody at 1/1,000 dilution.
  Positive control:
  Lane 1: HepG2
  Lane 2: K562
  Lane 3: HEK293

   

  

  Fig2: Immunohistochemical analysis of paraffin-embedded human muscle tissue using anti-MAP2K4 antibody. Counter stained with hematoxylin.

   

  

  Fig3: Flow cytometric analysis of K562 cells with MAP2K4 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; purple).

特别提示：本公司的所有产品仅可用于科研实验，严禁用于临床医疗及其他非科研用途！