Anti-Glucocorticoid Receptor alpha antibody [3F1]

Anti-Glucocorticoid Receptor alpha antibody [3F1]

• 概述

• 产品描述The glucocorticoid receptor (GR) is a ubiquitously expressed transcription factor that mediates the effects of glucocorticoids. The most abundant isoform is GR α. GR induces or represses the expression of genes in response to glucocorticoids, mediating such processes as apoptosis, cell growth and differentiation. A significant class of genes suppressed by GR is controlled by the transcription factor AP-1. GR has also been shown to be the limiting factor in the induction of gene expression by glucocorticoids. It has been revealed that GR forms a complex with HSP 90, rendering the non-ligand bound receptor transcriptionally inactive. More importantly, mutant GRs lacking the signaling domain remain constitutively active.
• 产品名称Anti-Glucocorticoid Receptor alpha antibody [3F1]
• 分子量86 kDa
• 种属反应性Human
• 验证应用WB,ICC,IHC-P,FC
• 抗体类型小鼠单抗
• 免疫原Recombinant full length protein corresponding to Human Glucocorticoid Receptor alpha.
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度2 mg/mL.
• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
• 存储缓冲液1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
• 亚型IgG2a
• 纯化方式Protein G purified.
• 亚细胞定位Nucleus. Cytoskeleton. Mitochondrion.
• 其它名称
  • GCCR antibody
  • GCR antibody
  • Glucocorticoid receptor alpha isoform antibody
  more

• 应用

  WB: 1:1,000-1:5,000
  ICC:1:50-1:200
  IHC-P: 1:50-1:200
  FC: 1:50-1:100

•  

  

  Fig1: Western blot analysis of Glucocorticoid Receptor alpha on SiHa (1) and A549 (2) cell lysates using anti-Glucocorticoid Receptor alpha antibody at 1/5,000 dilution.

   

  

  Fig2: ICC staining Glucocorticoid Receptor alpha (green) in SiHa cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

   

  

  Fig3: Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using anti-Glucocorticoid Receptor alpha antibody. Counter stained with hematoxylin.

   

  

  Fig4: Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-Glucocorticoid Receptor alpha antibody. Counter stained with hematoxylin.

   

  

  Fig5: Flow cytometric analysis of A549 cells with Glucocorticoid Receptor alpha antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-mouse IgG was used as the secondary antibody.

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