Anti-CD68 antibody [F3-D7]

Anti-CD68 antibody [F3-D7]

• 概述

• 产品描述CD68, which is homologous to the mouse antigen macrosialin, belongs to a family of acidic, highly glycosylated lysosomal glycoproteins (LGPs) that includes lamp-1 and lamp-2. CD68 is found in cytoplasmic granules and in the cytoplasm of various non-hematopoietic tissues including liver and kidney tubules and glomeruli. CD68 is also found, to a lesser extent, on the surface of macrophages, monocytes, neutrophils, basophils and large lymphocytes. LGPs are major components of lysosomal membranes and may act to protect the membranes from attack by hydrolases.
• 产品名称Anti-CD68 antibody [F3-D7]
• 分子量37 kDa
• 种属反应性Human
• 验证应用WB,IHC-P,ICC,FC
• 抗体类型小鼠单抗
• 免疫原Recombinant protein
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度2 mg/mL.
• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
• 存储缓冲液1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
• 亚型IgG1
• 纯化方式Protein A purified.
• 亚细胞定位Cell membrane and Endosome membrane. Lysosome membrane.
• 其它名称
  • CD 68 antibody
  • CD68 antibody
  • CD68 antigen antibody
  more

• 应用

  WB: 1:500-1:2,000
  ICC: 1:200-1:1,000
  IHC-P: 1:200-1:1,000
  FC: 1:50-1:100

•  

  

  Fig1: Western blot analysis of CD68 on human CD68 recombinant protein using anti-CD68 antibody at 1/1,000 dilution.

   

  

  Fig2: Western blot analysis of CD68 on HEK293 (1) and CD68-hIgGFc transfected HEK293 (2) cell lysate using anti-CD68 antibody at 1/1,000 dilution.

   

  

  Fig3: Western blot analysis of CD68 on different cell lysate using anti-CD68 antibody at 1/1,000 dilution.
  Positive control: Line1: U937 Line1: Hela Line2: HepG2 Line3: Jurkat

   

  

  Fig4: ICC staining CD68 (green) and Actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

   

  

  Fig5: Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue using anti-CD68 antibody. Counter stained with hematoxylin.

   

  

  Fig6: Immunohistochemical analysis of paraffin-embedded human bladder cancer tissue using anti-CD68 antibody. Counter stained with hematoxylin.

   

  

  Fig7: Flow cytometric analysis of Hela cells with CD68 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).

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