Anti-CTNNBL1 antibody [5E-4F]

Anti-CTNNBL1 antibody [5E-4F]

• 概述

• 产品描述CTNNBL1 (catenin, β like 1), also known as NAP (nuclear-associated protein) or P14L, is an evolutionarily conserved protein with structural homology to members of the armadillo family, including β-catenin. CTNNBL1 is expressed in a variety of tissues with highest expression levels found in heart, spleen, testis, placenta, thyroid and skeletal muscle. Localizing to the nucleus, CTNNBL1 contains a bipartite nuclear localization signal, an acidic domain, a leucine-isoleucine zipper, an acidic domain and phosphorylation sites. Via its C-terminus, CTNNBL1 is believed to play a role in apoptosis. In addition, multiple SNPs (single nucleotide polymorphisms) in the CTNNBL1 have been associated with fat mass and body mass index (BMI), suggesting a possible role for CTNNBL1 in the development of obesity.
• 产品名称Anti-CTNNBL1 antibody [5E-4F]
• 分子量65 kDa
• 种属反应性Human
• 验证应用WB,ICC,IHC-P,FC
• 抗体类型小鼠单抗
• 免疫原Recombinant protein
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度2 mg/mL.
• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
• 存储缓冲液1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
• 亚型IgG1
• 纯化方式Protein A purified.
• 亚细胞定位Nucleus.
• 其它名称
  • 5730471K09Rik antibody
  • Beta catenin like 1 antibody
  • Beta catenin like protein 1 antibody
  more

• 应用

  WB: 1:500-1:2,000
  ICC: 1:50-1:200
  IHC-P: 1:50-1:200
  FC: 1:50-1:100

•  

  

  Fig1: Western blot analysis of CTNNBL1 on human CTNNBL1 recombinant protein using anti-CTNNBL1 antibody at 1/1,000 dilution.

   

  

  Fig2: Western blot analysis of CTNNBL1 on HEK293 (1) and CTNNBL1-hIgGFc transfected HEK293 (2) cell lysate using anti-CTNNBL1 antibody at 1/1,000 dilution.

   

  

  Fig3: Western blot analysis of CTNNBL1 on different cell lysate using anti-CTNNBL1 antibody at 1/1,000 dilution.
  Positive control: Line1: Hela Line2: Jurkat Line3: HEK293 Line4: A431 Line5: HepG2 Line6: Raji

   

  

  Fig4: ICC staining CTNNBL1 (green) in MCF-7 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

   

  

  Fig5: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-CTNNBL1 antibody. Counter stained with hematoxylin.

   

  

  Fig6: Immunohistochemical analysis of paraffin-embedded human esophageal cancer tissue using anti-CTNNBL1 antibody. Counter stained with hematoxylin.

   

  

  Fig7: Flow cytometric analysis of Hela cells with CTNNBL1 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).

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