Anti-CD177 antibody [2F2C1]

Anti-CD177 antibody [2F2C1]

• 概述

• 产品描述This gene encodes a glycosyl-phosphatidylinositol (GPI)-linked cell surface glycoprotein that plays a role in neutrophil activation. The protein can bind platelet endothelial cell adhesion molecule-1 and function in neutrophil transmigration. Mutations in this gene are associated with myeloproliferative diseases. Over-expression of this gene has been found in patients with polycythemia rubra vera. Autoantibodies against the protein may result in pulmonary transfusion reactions, and it may be involved in Wegener's granulomatosis. A related pseudogene, which is adjacent to this gene on chromosome 19, has been identified.
• 产品名称Anti-CD177 antibody [2F2C1]
• 分子量46.4kDa
• 种属反应性Human
• 验证应用WB,FC
• 抗体类型小鼠单抗
• 免疫原Purified recombinant fragment of human CD177 (AA: extra 22-161) expressed in E. Coli.
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度1 mg/mL
• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
• 存储缓冲液1*PBS with 0.05% sodium azide.
• 亚型IgG2b
• 纯化方式Protein G purified.
• 亚细胞定位Cell membrane.
• 其它名称
  • CD177 antibody
  • CD177 antigen antibody
  • CD177 molecule antibody
  • CD177_HUMAN antibody
  • HNA-2a antibody
  • HNA2A antibody
  • Human neutrophil alloantigen 2a antibody
  • NB1 antibody
  • NB1 glycoprotein antibody
  • NB1 GP antibody
  • Polycythemia rubra vera 1 antibody
  • Polycythemia rubra vera protein 1 antibody
  • PRV-1 antibody
  • PRV1 antibody
  pick up

• 应用

  WB: 1:500-1:2,000
  FC: 1:100-1:200

•  

  

  Fig1: Western blot analysis of CD177 against human CD177 (AA: extra 22-161) recombinant protein. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody  was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.

   

  

  Fig2: Western blot analysis of CD177 against HEK293 (1) and CD177 (AA: extra 22-161)-hIgGFc transfected HEK293 (2) cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.

   

  

  Fig3: Western blot analysis of CD177 against SPC-A-1 (1), SK-MES-1 (2), HepG2 (3), HL-60 (4), and PC-3 (5) cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody  was used in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.

   

  

  Fig4: Flow cytometric analysis of CD177 was done on HL-60 cells . The cells were fixed, permeabilized and stained with the primary antibody (green). After incubation of the primary antibody at room temperature for an hour, the cells were stained with a Alexa Fluor 488-conjugated goat anti-Mouse IgG Secondary antibody at 1/500 dilution for 30 minutes. Unlabelled sample was used as a control (cells without incubation with primary antibody; red).

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