Anti-PD1 antibody [1F2]

Anti-PD1 antibody [1F2]

• 概述

• 产品描述Pdcd-1 (Programmed Cell Death-1 protein), also designated CD279, is a type I transmembrane receptor and a member of the immunoglobin gene superfamily. Pdcd-1 contains an immunoreceptor tyrosine-based inhibitory motif (ITIM) within the cytoplasmic domain, which is conserved between the mouse and human homologs. Expression of Pdcd-1 is detected in mouse thymus, and it is induced in stimulated B and T cell lines, where it may play a role in the negative regulation of various immune responses. Receptors such as Pdcd-1 function by recruiting tyrosine phosphatases, including SHP-1 and SHIP, which are responsible for altering various B cell responses. Additionally, in activated lymphocytes, Pdcd-1 mediates the activation of the classical type of programmed cell death.
• 产品名称Anti-PD1 antibody [1F2]
• 分子量32 kDa
• 种属反应性Human
• 验证应用WB,ICC,IHC-P,FC
• 抗体类型小鼠单抗
• 免疫原Recombinant protein
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度2 mg/mL.
• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
• 存储缓冲液1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
• 亚型IgG1
• 纯化方式Protein A purified.
• 亚细胞定位Membrane.
• 其它名称
  • CD279 antibody
  • CD279 antigen antibody
  • hPD 1 antibody
  more

• 应用

  WB: 1:500-1:2,000
  ICC: 1:50-1:200
  IHC-P: 1:100-1:500
  FC: 1:50-1:100

•  

  

  Fig1: Western blot analysis of PD1 on PD1 transfected 293FT cell lysate using anti-PD1 antibody at 1/1,000 dilution.

   

  

  Fig2: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-PD1 antibody. Counter stained with hematoxylin.

   

  

  Fig3: Flow cytometric analysis of Jurkat cells with PD1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).

特别提示：本公司的所有产品仅可用于科研实验，严禁用于临床医疗及其他非科研用途！