Anti-MuRF1 antibody [B8-E1]

Anti-MuRF1 antibody [B8-E1]

• 概述

• 产品描述Muscle specific RING finger protein (MuRF1) is a sarcomere-associated protein that is upregulated by conditions that provoke atrophy. Pharmacological or genetic inhibition of the IKKbeta/NF-kappaB/MuRF1 pathway reverses muscle atrophy, which presents MuRF as a target for clinical intervention. MuRF1 is a key regulator of the PKC-dependent hypertrophic response and can blunt cardiomyocyte hypertrophy, which may have important implications in the pathophysiology of clinical cardiac hypertrophy. MuRF1 directly associates with Titin kinase and influences microtubule-dependent signaling pathways in striated muscle and iris. MuRF1 upregulation is an indicator for skeletal muscle atrophy mechanisms that utilize ubiquitin-dependent proteolysis. MuRF1 transcript levels are high in situations where there is an overabundance of reactive oxygen species, such as cancer, AIDS and sepsis.
• 产品名称Anti-MuRF1 antibody [B8-E1]
• 分子量40 kDa
• 种属反应性Human
• 验证应用ICC,IHC-P,FC
• 抗体类型小鼠单抗
• 免疫原Recombinant protein
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度2 mg/mL.
• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.
• 存储缓冲液1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
• 亚型IgG1
• 纯化方式Protein A purified.
• 亚细胞定位Cytoplasm. Nucleus
• 其它名称
  • E3 ubiquitin-protein ligase TRIM63 antibody
  • FLJ32380 antibody
  • IRF antibody
  more

• 应用

  ICC: 1:50-1:200
  IHC-P: 1:50-1:200
  FC: 1:50-1:100

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  Fig1: ICC staining MuRF1(green) and Actin filaments(red) inHela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

   

  

  Fig2: Immunohistochemical analysis of paraffin-embedded human rectum cancer tissues using anti-MuRF1 antibody. Counter stained with hematoxylin.

   

  

  Fig3: Immunohistochemical analysis of paraffin-embedded huamn muscle tissues using anti-MuRF1 antibody. Counter stained with hematoxylin.

   

  

  Fig4: Flow cytometric analysis of Hela cells with MuRF1 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).

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