Anti-Ring1 antibody [F4-C12]

Anti-Ring1 antibody [F4-C12]

• 概述

• 产品描述In Drosophila, the Polycomb (PcG) gene family encodes chromatin proteins that are required for the repression of homeotic loci during embryonic development. The human PcG homologues form two distinct multimeric protein complexes, the EED/EZH protein complex and the HPC/HPH protein complex, which have mutually exclusive expression patterns. The HPC/HPH PcG complex contains the human polycomb 2 (HPC2), human polyhomeotic (HPH), Bmi-1 and RING1 proteins. The human RING1 gene, which is proximal to the major histocompatibility complex region on chromosome six, encodes for a protein that contains a RING finger motif, a zinc-binding domain found in many regulatory proteins, but unlike the other human PcG genes, RING1 displays no homology to known Drosophila PcG genes. RING1 strongly represses En-2, the mammalian homolog of the Drosophila engrailed gene, and when overexpressed, it mediates an increase in the expression of proto-oncogenes, such as c-Jun and c-fos. Also, loss of RING1 and Bmi-1 expression correlates with the differentiation of B cells, which suggests a role for RING1 in germinal center development.

• 产品名称Anti-Ring1 antibody [F4-C12]

• 分子量50 kDa

• 种属反应性Human

• 验证应用WB,IHC-P,FC

• 抗体类型小鼠单抗

• 免疫原Recombinant protein

• 偶联Non-conjugated

• 性能

• 形态Liquid

• 浓度2 mg/mL.

• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.

• 存储缓冲液1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.

• 亚型IgG1

• 纯化方式Protein A purified.

• 亚细胞定位Nucleus speckle.

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• ​其它名称

  more

  • Ring1A antibody

  • E3 ubiquitin-protein ligase RING1 antibody

  • Polycomb complex protein RING1 antibody

• 应用

  WB: 1:500-1:1,000
  IHC-P: 1:100-1:200
  FC: 1:100-1:200

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  Fig1: Western blot analysis of Ring1 on human Ring1 recombinant protein using anti-Ring1 antibody at 1/1,000 dilution.

   

  

  Fig2: Western blot analysis of Ring1 on HEK293 (1) and Ring1-hIgGFc transfected HEK293 (2) cell lysate using anti-Ring1 antibody at 1/1,000 dilution.

   

  

  Fig3: Western blot analysis of Ring1 on different cell lysate using anti-Ring1 antibody at 1/1,000 dilution.
  Positive control: Line1: MOLT-4 Line2: LNCaP Line3: Hela Line4: HEK-293 Line5: Jurkat

   

  

  Fig4: Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue using anti-Ring1 antibody. Counter stained with hematoxylin.

   

  

  Fig5: Immunohistochemical analysis of paraffin-embedded human rectum cancer tissue using anti-Ring1 antibody. Counter stained with hematoxylin.

   

  

  Fig6: Flow cytometric analysis of Hela cells with Ring1 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).

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