Anti-PPP2R4 antibody [G4-D9]

Anti-PPP2R4 antibody [G4-D9]

• 概述

• 产品描述n general, the protein phosphatase (PP) holoenzyme is a trimeric complex composed of a regulatory subunit, a variable subunit and a catalytic subunit. Four major families of protein phosphatase catalytic subunits have been identified, designated PP1, PP2A, PP2B (calcineurin) and PP2C. PPP2R4 (protein phosphatase 2A activator, regulatory subunit 4), also known as PR53 or PTPA, is a 358 amino acid protein that is widely expressed and associates with the PP holoenzyme. Specifically, PPP2R4 functions to stimulate the ATP- and magnesium-dependent phosphotyrosyl phosphatase activity of the dimeric form of PP2A, thereby affecting the control of cell growth and division. Four isoforms of PPP2R4, designated alpha, beta, delta and epsilon, are expressed due to alternative splicing events.

• 产品名称Anti-PPP2R4 antibody [G4-D9]

• 分子量41 kDa

• 种属反应性Human

• 验证应用WB,ICC,IHC-P,FC

• 抗体类型小鼠单抗

• 免疫原Recombinant protein

• 偶联Non-conjugated

• 性能

• 形态Liquid

• 浓度2 mg/mL.

• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃ or -80℃. Avoid repeated freeze / thaw cycles.

• 存储缓冲液1*TBS (pH7.4), 1%BSA, Preservative: 0.05% Sodium Azide.

• 亚型IgG1

• 纯化方式Protein A purified.

• 亚细胞定位Cytoplasm. Nucleus.

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• ​其它名称

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  • subunit B antibody

  • KIAA0044 antibody

  • MGC2184 antibody

• 应用

  WB: 1:500-1:1,000
  ICC: 1:100-1:500
  IHC-P: 1:100-1:500
  FC: 1:100-1:200

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  Fig1: Western blot analysis of PPP2R4 on human PPP2R4 recombinant protein using anti-PPP2R4 antibody at 1/1,000 dilution.

   

  

  Fig2: ICC staining PPP2R4 (green) and actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

   

  

  Fig3: Immunohistochemical analysis of paraffin-embedded cervical cancer tissue using anti-PPP2R4 antibody. Counter stained with hematoxylin.

   

  

  Fig4: Immunohistochemical analysis of paraffin-embedded lung cancer tissue using anti-PPP2R4 antibody. Counter stained with hematoxylin.

   

  

  Fig5: Flow cytometric analysis of MCF-7 cells with PPP2R4 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).

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