Anti-MMP-3 antibody [3G1]

Anti-MMP-3 antibody [3G1]

• 概述

• 产品描述The matrix metalloproteinases (MMP) are a family of peptidase enzymes responsible for the degradation of extracellular matrix components, including collagen, gelatin, fibronectin, laminin and proteoglycan. Transcription of MMP genes is differentially activated by phorbol ester, lipopolysaccharide (LPS) or staphylococcal enterotoxin B (SEB). MMP catalysis requires both calcium and zinc. MMP-3, MMP-10 and MMP-11 (also designated stromelysin-1, -2 and -3 respectively) activate procollagenase. MMP-3 activation of procollagenase can occur via two pathways. Direct activation by MMP-3 is slow and activation by MMP-3 in conjunction with tissue or plasma proteinases is rapid. MMP-10 is expressed in small intestine, and at lower levels in lung and heart. MMP-11 is specifically expressed in stromal cells of breast carcinomas and contributes to epithelial cell malignancies.
• 产品名称Anti-MMP-3 antibody [3G1]
• 分子量57 kDa
• 种属反应性Human
• 验证应用WB,IP,IF,IHC-P
• 抗体类型小鼠单抗
• 免疫原Amino acids 317-327 of MMP-3 of human origin.
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度2 mg/mL.
• 存放说明Store at +4℃
• 存储缓冲液1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
• 亚型IgG1
• 纯化方式Protein A purified.
• 亚细胞定位Secreted
• 其它名称
  • CHDS6 antibody
  • Matrix metalloproteinase 3 antibody
  • Matrix metalloproteinase-3 antibody
  more

• 应用

  WB: 1:100-1:1,000
  IP: 1-2 μg per 100-500 μg of total protein(1 ml of cell lysate)
  IF: 1:50-1:500
  IHC-P: 1:50-1:500

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  Fig1: Western blot analysis of MMP-3 expression in HT-1080 (A), ECV304 (B), HUVEC-C (C), WI38 (D), BJ (E) and CCD-1064SK (F) whole cell lysates.

   

  

  Fig2: Immunofluorescence staining of methanol-fixed Y-79 cells showing membrane staining (A). Immunoperoxidase staining of formalin fixed, paraffin-embedded human prostate tissue showing cytoplasmic staining of glandular cells.(B).

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