Anti-Myeloperoxidase antibody [2G3]

Anti-Myeloperoxidase antibody [2G3]

• 概述

• 产品描述The heme protein myeloperoxidase (MPO) is a major component of azurophilic granules of neutrophils and polymorphonuclear leukocytes. Optimal oxygen-dependent microbiocidal activity depends on MPO as the critical enzyme for the generation of hypochlorous acid and other toxic oxygen products. The MPO precursor is synthesized during the promyelocytic stage of myeloid differentiation and is subsequently processed and transported intracellularly to the lysosomes. The precursor undergoes cotranslational N-linked glycosylation to produce a glycoprotein. Glucosidases in the endoplasmic reticulum (ER) or early cis Golgi convert the pro-MPO to a form which is sorted into a prelysosomal compartment, which undergoes final proteolytic maturation to native MPO, a pair of heavy-light protomers. In normal neutrophils, MPO is expressed as a dimer. Calreticulin, a calcium-binding protein residing in the ER, interacts specifically with fully glycosylated apopro-MPO. iMPO mRNA is abundant in human promyelocytic HL-60 and mouse myeloid leukemia NFS-60 cells. MPO is expressed at high levels in circulating neutrophils and monocytes but is not detectable in microglia, brain-specific macrophages or normal brain tissue.
• 产品名称Anti-Myeloperoxidase antibody [2G3]
• 分子量72 kDa
• 种属反应性Human
• 验证应用WB,IP,IF
• 抗体类型小鼠单抗
• 免疫原Immunogen not available.
• 偶联Non-conjugated

• 性能

• 形态Liquid
• 浓度2 mg/mL.
• 存放说明Store at +4℃
• 存储缓冲液1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
• 亚型IgG1
• 纯化方式Protein A purified.
• 亚细胞定位Lysosome
• 其它名称
  • 84 kDa myeloperoxidase antibody
  • 89 kDa myeloperoxidase antibody
  • EC 1.11.1.7 antibody
  more

• 应用

  WB: 1:100-1:1,000
  IP: 1-2 μg per 100-500 μg of total protein(1 ml of cell lysate)
  IF: 1:50-1:500

•  

  

  Fig1: Western blot analysis of MPO expression in HL-60 whole cell lysate.

   

  

  Fig2: Immunofluorescence staining of methanol-fixed HL-60 cells showing nuclear localization.

特别提示：本公司的所有产品仅可用于科研实验，严禁用于临床医疗及其他非科研用途！