Mouse anti-Human IgG heavy chain-HRP antibody [54-76]

Mouse anti-Human IgG heavy chain-HRP antibody [54-76]

• 概述

• 产品描述Whole IgG antibodies are isolated as intact molecules from antisera by immunoaffinity chromatography. They have an Fc portion and two antigen binding Fab portions joined together by disulfide bonds and therefore they are divalent. The average molecular weight is reported to be about 160 kDa. The whole IgG form of antibodies is suitable for the majority of immunodetection procedures and is the most cost effective. Horseradish peroxidase (HRP) conjugates are prepared by a modified Nakane and Kawaoi procedure (J. Histochem. Cytochem. 1974. 22, 1084). Peroxidase conjugates are commonly used for immunohistochemistry, Western blotting, and ELISA. Affinity-purified anti-horseradish peroxidase and conjugates are available for detection of horseradish peroxidase antigen or for signal amplification of HRP-containing reagents. For immunostaining of mammalian cells, an advantage of using anti-horseradish peroxidase is reduced background, since the antibody does not recognize the endogenous peroxidase-like enzymes found in those cells.
• 产品名称Mouse anti-Human IgG heavy chain-HRP antibody [54-76]
• 分子量50 kDa
• 种属反应性Human
• 验证应用WB, ELISA
• 抗体类型小鼠单抗
• 免疫原Human IgG
• 偶联HRP-conjugated

• 性能

• 形态Liquid
• 浓度2 mg/mL.
• 存放说明Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.
• 存储缓冲液1*PBS (pH7.4), 0.2% BSA, 40% Glycerol.
• 亚型IgG2a
• 纯化方式Affinity purified
• 亚细胞定位serum plasma

• 应用

  WB: 1:5,000
  ELISA: 1:5,000

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  Fig1: Western blot analysis of Mouse anti-Human IgG heavy chain-HRP on human serum lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used in 5% BSA at room temperature for 2 hours.

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