低氧诱导因子1α(HIF1a)重组蛋白(Pig，猪)

[ PROPERTIES ]

Source: Prokaryotic expression

Host: E.coli

Residues: Glu585~Asn824, with two N-terminal Tags, His-tag and GST-tag.

Tags: N-terminal His Tag

Subcellular Location: Secreted

Purity: >95%

Endotoxin Level: <1.0EU per 1μg (determined by the LAL method).

Formulation: Supplied as lyophilized form in PBS, pH7.4, containing 5% sucrose, 0.01% sarcosyl.

Applications: SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

Predicted isoelectric point: 6.0

Predicted Molecular Mass: 57.9kDa

[ USAGE ]

Reconstitute in sterile PBS, pH7.2-pH7.4.

[ STORAGE AND STABILITY ]

Storage:Avoid repeated freeze/thaw cycles.

Store at 2-8°C for one month.

Aliquot and store at -80°C for 12 months.

Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.

[ SEQUENCES ]

The target protein is fused with two N-terminal Tags, His-tag and GST-tag, its sequence is listed below.

MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID

GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV

DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK

KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPR

GSTAIGMKET AAAKFERQHM DSPDLGTLEV LFQGPLGSEF- ESSSTS PQSTSTITVF

QPTPMPEPPV TTTTPAATTD ELKTVAKDGM EDIKILIAFP SPPHVHKETT CATTSPYSDT

GSRTASPNRA GKGVIEQTEK SHPRSPNVLS VALSQRTTVP EEELNPKIIA FQNAQRKRKI

EHDGSLFQAV GIGTLLQQPD DRATTTSLSW KRVKGCKSSE QNGTEQKTII LIPSDLACRL

LGQSMDESGL PQLTSYDCEV NAPIQGSRNL 

[ IDENTIFICATION ]

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