组织蛋白酶K(CTSK)重组蛋白(Simian，恒河猴)

[ PROPERTIES ]

Host: E.coli

Residues: Pro116~Met329 , with N-terminal His-Tag.

Subcellular Location: Lysosome

Purity: >95%

Endotoxin Level: <1.0EU per 1μg (determined by the LAL method).

Formulation: Supplied as lyophilized form in PBS, pH7.4, containing 5% sucrose, 0.01% sarcosyl.

Applications: SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

Predicted isoelectric point: 8.8

Predicted Molecular Mass: 24.9kDa

[ USAGE ]

Reconstitute in sterile PBS, pH7.2-pH7.4.

[ STORAGE AND STABILITY ]

Storage:Avoid repeated freeze/thaw cycles.

Store at 2-8°C for one month.

Aliquot and store at -80°C for 12 months.

Stability Test: 

The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.

[ SEQUENCES ]

The target protein is fused with N-terminal His-Tag, its sequence is listed below.

MGHHHHHHSGSEF-PDSVD YRKKGYVTPV KNQGQCGSCW AFSSVGALEG QLKKKTGKLL

NLS PQ N LV D C VSE ND G CG G G YM T NAFQYV Q KNR GI DSE DA YPYV GQE ES C

MYNPTGKAAK CRGYREIPEG NEKALKRAVA RVGPVSVAID ASLTSFQFYS KGVYYDESCN

SDNLNHAVLA VGYGIQKGNK HWIIKNSWGE NWGNKGYILM ARNKNNACGI ANLASFPKM

[ IDENTIFICATION ]

[ REFERENCES ]

1. Guay J., et al. (1999) Bone 25:205-209.
2. Yamashita D.S., et al. (2006) J. Med. Chem. 49:1597-1612.
3. Motyckova G., Fisher DE. (2003) Curr. Mol. Med. 2 (5): 407–21.

4. Troen BR. (2006) Ann. N. Y. Acad. Sci. 1068: 165–72

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