[ PROPERTIES ]
Source: Prokaryotic expression
Host: E.coli
Residues: Val19~Pro158 , with two N-terminal Tags, His-tag and GST-tag.
Subcellular Location: Secreted
Purity: >95%
Endotoxin Level: <1.0EU per 1μg (determined by the LAL method). Formulation: Supplied as lyophilized form in 20mM Tris, 500mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose, and preservative.
Applications: SDS-PAGE; WB; ELISA; IP.
(May be suitable for use in other assays to be determined by the end user.)
Predicted isoelectric point: 6.3
Predicted Molecular Mass: 47.9kDa
Accurate Molecular Mass: 38&46kDa as determined by SDS-PAGE reducing conditions.
Phenomenon explanation:
The possible reasons that the actual band size differs from the predicted are as follows:
1.Splice variants: Alternative splicing may create different sized proteins from the same gene.
2. Relative charge: The composition of amino acids may affects the charge of the protein.
3. Post-translational modification: Phosphorylation, glycosylation, methylation etc.
4. Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleavedto give the active form.
5. Polymerization of the target protein: Dimerization, multimerization etc.
[ USAGE ]
Reconstitute in sterile PBS, pH7.2-pH7.4.
[ STORAGE AND STABILITY ]
Storage:Avoid repeated freeze/thaw cycles.
Store at 2-8°C for one month.
Aliquot and store at -80°C for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
[ SEQUENCES ]
The target protein is fused with two N-terminal Tags, His-tag and GST-tag, its sequence is listed below.
MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID
GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV
DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK
KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPR
GSTAIGMKET AAAKFERQHM DSPDLGTLEV LFQGPLGSEF -VH SQGALSAGNN
STEVEVVPFN EVWGRSYCRP MEKLVYILDE YPDEVSHIFS PSCVLLSRCS GCCGDEGLHC
VPIKTANITM QILKIPPNRD PHFYVEMTFS QDVLCECRPI LETTKAERRK TKGKRKRSRN
SQTEEPHP
[ REFERENCES ]
1. Dipalma T., et al. (1996) Mamm. Genome 7:6-12.
2. Achen M.G., et al. (1997) Growth Factors 15:69-80.
3. Adini A., et al. (2002) Cancer Res. 62:2749-2752.
4. Luttun A., et al. (2002) Biochem. Biophys. Res. Commun. 295:428-434.
[ IDENTIFICATION ]
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