[ PROPERTIES ]
Host: E.coli
Residues: Ser18~Val372 , with two N-terminal Tags, His-tag and GST-tag.
Subcellular Location: Cell membrane; Lipid-anchor, GPI-anchor
Purity: >95%
Endotoxin Level: <1.0EU per 1μg (determined by the LAL method).
Formulation: Supplied as lyophilized form in PBS, pH7.4, containing 5% sucrose, 0.01% sarcosyl.
Applications: SDS-PAGE; WB; ELISA; IP.
(May be suitable for use in other assays to be determined by the end user.)
Predicted isoelectric point: 5.7
Predicted Molecular Mass: 69.9kDa
[ USAGE ]
Reconstitute in sterile PBS, pH7.2-pH7.4.
[ STORAGE AND STABILITY ]
Storage:Avoid repeated freeze/thaw cycles.
Store at 2-8°C for one month.
Aliquot and store at -80°C for 12 months.
Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
[ SEQUENCES ]
The target protein is fused with two N-terminal Tags, His-tag and GST-tag, its sequence is listed below.
MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYID
GDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKV
DFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFK
KRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPR
GSTAIGMKET AA AKFE RQ HM DSP D LG T LEV LFQ GP LGS -SPA TPE P CE L DQ D
EESVRCYCNF SDPQPNWSSA FLCAGAEDVE FYGGGRSLEY LLKRVDTEAN LGQYTDIIRS
LPLKRLTVRS ARVPTQILFG TLRVLGYSGL RELTLENLEV TGTALSPLLD ATGPDLNTLS
LRNVSWATTD TWLAELQQWL KPGLKVLSIA QAHSLNFSCK QVGVFPALAT LDLSDNPELG
EKGLISALCP HKFPTLQVLA LRNAGMETTS GVCSALAAAR VPLQALDLSH NSLRDTAGTP
SCDWPSQLNS LNLSFTGLEH VPKGLPAKLS VLDLSYNRLD RKPRPEELPE VGSLSLTGNP
FLHSESQSEA YNSGVVIATA LSPGSAGLSG TLALLLGHRL FV
[ IDENTIFICATION ]
特别提示：本公司的所有产品仅可用于科研实验，严禁用于临床医疗及其他非科研用途！以实际收货产品说明书为准，网站说明书仅供参考。
