[ PROPERTIES ]
Source: Prokaryotic expression
Host: E.coli
Residues: Lys113~Lys283
Tags: N-terminal His Tag
Tissue Specificity: Spleen, Heart, Marrow.
Subcellular Location: Cell membrane; Single-pass type II membrane protein, Secreted.
Purity: >92%
Traits: Freeze-dried powder
Buffer formulation: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5%Trehalose and Proclin300.
Original Concentration: 200ug/mL
Applications: SDS-PAGE; WB; ELISA; IP; CoIP; Purification; Amine Reactive Labeling.
(May be suitable for use in other assays to be determined by the end user.)
Predicted isoelectric point: 5.9
Predicted Molecular Mass: 20.4kDa
Accurate Molecular Mass: 22kDa as determined by SDS-PAGE reducing conditions.
[ USAGE ]
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
[ STORAGE AND STABILITY ]
Storage:Avoid repeated freeze/thaw cycles.
Store at 2-8°C for one month.
Aliquot and store at -80°C for 12 months.
Stability Test: The thermal stability is described by the loss rate. The loss rate was determinedby accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and noobvious degradation and precipitation were observed. The loss rate is less than 5% within theexpiration date under appropriate storage condition.
[ SEQUENCE ]
[ IDENTIFICATION ]
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