[ PROPERTIES ]
Host: E.coli
Residues: Met1~Ala128 , with two N-terminal Tags, His-tag and GST-tag.
Subcellular Location: Cytoplasm
Purity: >95%
Endotoxin Level: <1.0EU per 1μg (determined by the LAL method).
Formulation: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
Applications: SDS-PAGE; WB; ELISA; IP.
(May be suitable for use in other assays to be determined by the end user.)
Predicted isoelectric point: 6.1
Predicted Molecular Mass: 46.2kDa
[ USAGE ]
Reconstitute in sterile PBS, pH7.2-pH7.4.
[ STORAGE AND STABILITY ]
Storage:Avoid repeated freeze/thaw cycles.
Store at 2-8°C for one month.
Aliquot and store at -80°C for 12 months.
Stability Test: The thermal stability is described by the loss rate of thetarget protein. The loss rate was determined by accelerated thermal degradationtest, that is, incubate the protein at 37oC for 48h, and no obvious degradationandprecipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this proteinislessthan 5% within the expiration date under appropriate storage condition.
[ SEQUENCES ]
The target protein is fused with two N-terminal Tags, His-tag and GST-tag, itssequence is listed below.
MSPILGYWKI KGLVQPTRLL LEYLEEKYEE HLYERDEGDK WRNKKFELGL EFPNLPYYIDGDVKLTQSMA IIRYIADKHN MLGGCPKERA EISMLEGAVL DIRYGVSRIA YSKDFETLKVDFLSKLPEML KMFEDRLCHK TYLNGDHVTH PDFMLYDALD VVLYMDPMCL DAFPKLVCFKKRIEAIPQID KYLKSSKYIA WPLQGWQATF GGGDHPPKSD GSTSGSGHHH HHHSAGLVPRGSTAIGMKET AAAKFERQHM DSPDLGTLEV LFQGPLGS-MAFTGKYEFE SEKNYDEFMKRLGLPDEVIE RGRNFKIITE VQQDGENFTW SQSYSGGNIM SNKFTIGKEC EMQTMGGKKFKATVKMEGGK VVADFPNYHQ TSEVVGDKLV EISTIGDVTY ERVSKRVA
[ IDENTIFICATION ]
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