Cynomolgus LRRC32&TGF-beta 1 Heterotrimer Protein, His Tag&Tag Free

Cynomolgus LRRC32&TGF-beta 1 Heterotrimer Protein, His Tag&Tag Free

分子别名（Synonym）

LRRC32 & TGF-beta 1,LRRC32&TGFB1

表达区间及表达系统（Source）

Cynomolgus LRRC32&TGF-beta 1 Heterotrimer Protein, His Tag&Tag Free is expressed from human 293 cells. It contains AA Ala 18 -Asn 627 (LRRC32) & Leu 30 - Ser 390 (TGF-beta 1).

Predicted N-terminus: Ala 18 (LRRC32) & Leu 30 (TGF-beta 1)

Request for sequence

蛋白结构（Molecular Characterization）

Cynomolgus LRRC32&TGFB1 Heterotrimer protein, His Tag&Tag Free (the molar ratio of LRRC32 & TGF-beta 1 equals 1:2) is produced by co-expression of LRRC32 and TGF-beta 1, which has a calculated MW of 68.1 kDa (LRRC32), 28.5 (LAP) and 12.8 kDa (mature TGF-beta 1) respectively. LRRC32 is fused with a polyhistidine tag at the C-terminus and TGF-beta 1 contains no tag. The reducing (R) Heterotrimer protein migrates as 70 kDa (LRRC32), 37-43 kDa (LAP) and 13 kDa (mature TGF-beta 1) due to glycosylation respectively.

内毒素（Endotoxin）

Less than 1.0 EU per μg by the LAL method.

纯度（Purity）

>90% as determined by SDS-PAGE.

制剂（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

1. -20°C to -70°C for 12 months in lyophilized state;

2. -70^°C for 3 months under sterile conditions after reconstitution.

电泳（SDS-PAGE）

Cynomolgus LRRC32&TGF-beta 1 Heterotrimer Protein, His Tag&Tag Free on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

活性（Bioactivity）-ELISA

Immobilized Cynomolgus LRRC32&TGF-beta 1 Heterotrimer Protein, His Tag&Tag Free  at 1 μg/mL (100 μL/well) can bind Biotinylated Human ITGAV&ITGB6 Heterodimer Protein, His,Avitag&Tag Free  with a linear range of 1-39 ng/mL (QC tested).

Protocol

Immobilized Cynomolgus LRRC32&TGF-beta 1 Heterotrimer Protein, His Tag&Tag Free  at 1 μg/mL (100 μL/well) can bind Mouse IgG1:Purified anti-human LAP(TGF-β1) with a linear range of 0.5-8 ng/mL (Routinely tested).

Protocol

活性（Bioactivity）-SPR

Anti-LRRC32 antibody captured on Protein A Chip can bind Cynomolgus LRRC32&TGF-beta 1 Heterotrimer Protein, His Tag&Tag Free with an affinity constant of 51.9 pM as determined in SPR assay (Biaco

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