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| 别名: | FGF R3 (IIIb),FGF R3B,CD333 | ||
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| 储存条件: | -20℃ | ||
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| 货号 | 规格 | 可用库存 | 销售价(RMB) | 您的折扣价(RMB) | 购买数量 |
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| 熔点: | |
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| 密度: | |
| 储存条件: | -20℃ |
Human FGF R3 (IIIb) / FGF R3B / CD333 Protein, Fc Tag (MALS verified)
FGF R3 (IIIb),FGF R3B,CD333
Human FGF R3 (IIIb), Fc Tag is expressed from human 293 cells . It contains AA Glu 23 - Gly 377
Predicted N-terminus: Glu 23
Request for sequence
This protein carries a human IgG1 Fc tag at the C-terminus.
The protein has a calculated MW of 65.0 kDa. The protein migrates as 80-90 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Less than 1.0 EU per μg by the LAL method.
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.
Human FGF R3 (IIIb), Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
The purity of Human FGF R3 (IIIb), Fc Tag is more than 90% and the molecular weight of this protein is around 132-162 kDa verified by SEC-MALS.
Report
Immobilized Human FGF basic, Tag Free at 5 μg/mL (100 μL/well) can bind Human FGF R3 (IIIb), Fc Tag with a linear range of 2-39 ng/mL (QC tested).
Protocol
Immobilized Biotinylated Human FGF basic, Avitag,His Tag at 1 μg/mL (100 μL/well) on streptavidinprecoated (0.5 μg/well) plate can bind Human FGF R3 (IIIb), Fc Tagwith a linear range of 1-39 ng/mL (Routinely tested).
Protocol
Human FGF R3 (IIIb), Fc Tag immobilized on CM5 Chip can bind Human FGF acidic, Tag Free with an affinity constant of 5.91 μM as determined in a SPR assay (Biacore 8K) (Routinely tested).
