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Human IL-18BP Protein, Fc Tag (MALS verified)

Human IL-18BP Protein, Fc Tag (MALS verified)
别名: IL18BP,IL18BPa,Tadekinig-alfa
储存条件: -20℃ 
Human IL-18BP Protein, Fc Tag (MALS verified)
货号 规格 可用库存 销售价(RMB) 您的折扣价(RMB) 购买数量
ZY-ILP-H5253-200ug 200ug 现货 2900.00 2900
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熔点:
密度:
储存条件: -20℃

 

Human IL-18BP Protein, Fc Tag (MALS verified)

分子别名(Synonym)

IL18BP,IL18BPa,Tadekinig-alfa

表达区间及表达系统(Source)

Human IL-18BP, Fc Tag  expressed from human 293 cells. It contains AA Thr 31 - Gly 194 .

Predicted N-terminus: Thr 31

Request for sequence

蛋白结构(Molecular Characterization)

This protein carries a human IgG1 Fc tag at the C-terminus.

The protein has a calculated MW of 44.3 kDa. The protein migrates as 56-66 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;

  2. -70°C for 3 months under sterile conditions after reconstitution.

 

电泳(SDS-PAGE)

Human IL-18BP, Fc Tag (Cat. No. ILP-H5253) SDS-PAGE gel

Human IL-18BP, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

The purity of Human IL-18BP, Fc Tag is more than 85% and the molecular weight of this protein is around 115-135 kDa verified by SEC-MALS.

Report

活性(Bioactivity)-SPR

Biotinylated Human SPR

Human IL-18BP, Fc Tag captured on CM5 chip via Anti-human IgG Fc antibodies surface can bind Human IL-18, Tag Free with an affinity constant of 1.71 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).

 

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