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| 别名: | IL2RA,CD25,p55,IL2-RA,IL-2-RA | ||
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| 储存条件: | -20℃ | ||
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| 货号 | 规格 | 可用库存 | 销售价(RMB) | 您的折扣价(RMB) | 购买数量 |
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| 熔点: | |
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| 密度: | |
| 储存条件: | -20℃ |
Human IL-2 R alpha / CD25 Protein, Fc Tag (MALS verified)
IL2RA,CD25,p55,IL2-RA,IL-2-RA
Human IL-2 R alpha Protein, Fc Tag is expressed from human 293 cells. It contains AA Glu 22 - Cys 213
Predicted N-terminus: Glu 22
Request for sequence
This protein carries a human IgG1 Fc tag at the C-terminus.
The protein has a calculated MW of 48.4 kDa. The protein migrates as 60-66 kDa under reducing (R) condition due to glycosylation.
Less than 1.0 EU per μg by the LAL method.
>95% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.
Human IL-2 R alpha Protein, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
The purity of Human IL-2 R alpha Protein, Fc Tag is more than 90% and the molecular weight of this protein is around 102-124 kDa verified by SEC-MALS.
Report
Immobilized Human IL-2 R alpha, Fc Tag at 2μg/mL (100 μL/well) can bind Human IL-2, Tag Free with a linear range of 0.2-1.56 ng/mL
Protocol
Loaded Human IL-2 R alpha, Fc Tag on Protein A Biosensor, can bind Human IL-2, Tag Free with an affinity constant of 43.2 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
