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Human IL-6 R alpha / CD126 Protein, His Tag

Human IL-6 R alpha / CD126 Protein, His Tag
别名: IL6R,CD126,IL-6R-1,IL-6RA,IL6RA,IL-6R-alpha,IL6RQ,gp80
储存条件: -20℃ 
Human IL-6 R alpha / CD126 Protein, His Tag
货号 规格 可用库存 销售价(RMB) 您的折扣价(RMB) 购买数量
ZY-ILR-H4223-50ug 50ug 现货 3150.00 3150
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熔点:
密度:
储存条件: -20℃

 

Human IL-6 R alpha / CD126 Protein, His Tag

分子别名(Synonym)

IL6R,CD126,IL-6R-1,IL-6RA,IL6RA,IL-6R-alpha,IL6RQ,gp80

表达区间及表达系统(Source)

Human IL-6 R alpha, His Tag  is expressed from human 293 cells . It contains AA Leu 20 - Pro 365.

Predicted N-terminus: Leu 20

Request for sequence

蛋白结构(Molecular Characterization)

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 39.4 kDa. The protein migrates as 55-75 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;

  2. -70°C for 3 months under sterile conditions after reconstitution.

 

电泳(SDS-PAGE)

Human IL-6 R alpha, His Tag (Cat. No. ILR-H4223) SDS-PAGE gel

Human IL-6 R alpha, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

 

活性(Bioactivity)-ELISA

Biotinylated Human ELISA

Immobilized ActiveMax® Human IL-6, Tag Freeat 5 μg/mL (100 μL/well) can bind Human IL-6 R alpha, His Tag with a linear range of 0.08-0.6 μg/mL (QC tested).

Protocol

活性(Bioactivity)-BLI

Biotinylated Human BLI

Loaded Human IL-6 R alpha, His Tag on HIS1K Biosensor, can bind ActiveMax® Human IL-6, Tag Free with an affinity constant of 20.3 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).

 

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