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| 别名: | IL-12 R beta 1,IL12RB1,CD212,IL12R,IL12RB,IL-12RB1 | ||
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| 储存条件: | -20℃ | ||
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| 货号 | 规格 | 可用库存 | 销售价(RMB) | 您的折扣价(RMB) | 购买数量 |
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| 熔点: | |
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| 密度: | |
| 储存条件: | -20℃ |
Human IL-12 R beta 1 / CD212 Protein, Fc Tag
IL-12 R beta 1,IL12RB1,CD212,IL12R,IL12RB,IL-12RB1
Human IL-12 R beta 1, Fc Tag is expressed from human 293 cells . It contains AA Cys 24 - Glu 540
Predicted N-terminus: Cys 24
Request for sequence
This protein carries a human IgG1 Fc tag at the C-terminus.
The protein has a calculated MW of 83.5 kDa. The protein migrates as 90 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
Less than 1.0 EU per μg by the LAL method.
>95% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in Tris with Glycine, Arginine and NaCl, pH7.5. Normally trehalose is added as protectant before lyophilization.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
-20°C to -70°C for 12 months in lyophilized state;
-70°C for 3 months under sterile conditions after reconstitution.
Human IL-12 R beta 1, Fc Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
获取HPLC数据
Immobilized Human IL-12B, His Tag at 5 μg/mL (100 μL/well) can bind Human IL-12 R beta 1, Fc Tag with a linear range of 5-78 ng/mL (QC tested).
Protocol
Human IL-12 R beta 1, Fc Tagcaptured on CM5 chip via Anti-human IgG Fc antibodies surface can bind Human IL-12B&IL-12A Heterodimer Protein, His Tag&Flag Tag with an affinity constant of 2.06 nM as determined in a SPR assay (Biacore T200) (Routinely tested).
Protocol
Loaded Human IL-12 R beta 1, Fc Tagon Protein A Biosensor, can bind Human IL-12B, His Tag with an affinity constant of 7.14 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
Protocol
Loaded Human IL-12B&IL-12A Heterodimer Protein, His Tag&Flag Tagon HIS1K Biosensor, can bind Human IL-12 R beta 1, Fc Tag with an affinity constant of 32.3 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
