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ActiveMax® Human VEGF121 Protein, Tag Free

ActiveMax® Human VEGF121 Protein, Tag Free
别名: RP1-261G23.1,MGC70609,MVCD1,VEGFA,VPF
储存条件: -20℃ 
ActiveMax® Human VEGF121 Protein, Tag Free
货号 规格 可用库存 销售价(RMB) 您的折扣价(RMB) 购买数量
ZY-VE1-H4213-20ug 20g 现货 1980.00 1980
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熔点:
密度:
储存条件: -20℃

 

Biotinylated Human VEGF121 Protein, Avitag™,His Tag

分子别名(Synonym)

RP1-261G23.1,MGC70609,MVCD1,VEGFA,VPF

表达区间及表达系统(Source)

ActiveMax® Human VEGF121, Tag Free  is expressed from human 293 cells . It contains AA Ala 27 - Arg 147 .

Predicted N-terminus: Ala 27

Request for sequence

蛋白结构(Molecular Characterization)

This protein carries no "tag".

The protein has a calculated MW of 14.1 kDa. As a result of glycosylation, the protein migrates as 15-16 kDa and 17-20 kDa under reducing (R) condition, and 30-40 kDa under non-reducing (NR) condition (SDS-PAGE).

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>95% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;

  2. -70°C for 3 months under sterile conditions after reconstitution.

 

电泳(SDS-PAGE)

ActiveMax® Human VEGF121, Tag Free (Cat. No. VE1-H4213) SDS-PAGE gel

ActiveMax® Human VEGF121, Tag Free on SDS-PAGE under reducing (R) and non-reducing (NR) conditions. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

 

活性(Bioactivity)-ELISA

Biotinylated Human ELISA

Immobilized ActiveMax® Human VEGF121, Tag Free at 2 μg/mL (100 μL/well) can bind Human VEGF R2, Strep Tag ith a linear range of 0.15-2.5 μg/mL (QC tested).

 

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