PE-Labeled Human EGF R Protein, His Tag (Site-specific conjugation)

PE-Labeled Human EGF R Protein, His Tag (Site-specific conjugation)

分子别名（Synonym）

EGFR,ERBB,ERBB1,HER1,PIG61,mENA

表达区间及表达系统（Source）

PE-Labeled Human EGF R, His Tag  is produced via site-specific conjugation of PE to Human EGF R, His Tag under optimal conditions with a proprietary technology. Human EGF R, His Tag is expressed from human 293 cells ). It contains AA Leu 25 - Ser 645 .

Predicted N-terminus: Leu 25

Request for sequence

蛋白结构（Molecular Characterization）

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 72.3 kDa.

应用说明（Application）

Evaluation of anti-EGF R CAR expression by flow cytometry (Please note that this product is NOT compatible to streptavidin detection system).

Conjugate

PE

Excitation Wavelength: 488 nm / 561 nm

Emission Wavelength: 575 nm

制剂（Formulation）

Lyophilized from 0.22 μm filtered solution in PBS, 0.5% BSA, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法（Reconstitution）

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储（Storage）

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please protect from light and avoid repeated freeze-thaw cycles.

This product is stable after storage at:

1. -20°C to -70°C for 12 months in lyophilized state;

2. -70°C for 3 months under sterile conditions after reconstitution.

活性（Bioactivity）-FACS

5e5 of anti-EGFR CAR-293 cells were stained with 100 μL of 1:50 dilution (2 μL stock solution in 100 μL FACS buffer) of PE-Labeled Human EGF R, His Tag  and negative control protein respectively. PE signal was used to evaluate the binding activity (QC tested).

特别提示：本公司的所有产品仅可用于科研实验，严禁用于临床医疗及其他非科研用途！