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Cynomolgus / Rhesus macaque TNFR2 / CD120b / TNFRSF1B Protein, His Tag

Cynomolgus / Rhesus macaque TNFR2 / CD120b / TNFRSF1B Protein, His Tag
别名: TNFRSF1B,CD120b,TBPII,TNF-R-II,TNF-R75,TNFBR,TNFR1B,TNFR2,TNFR80,p75,p75TNFR
储存条件: -20℃ 
Cynomolgus / Rhesus macaque TNFR2 / CD120b / TNFRSF1B Protein, His Tag
货号 规格 可用库存 销售价(RMB) 您的折扣价(RMB) 购买数量
ZY-TN2-C52H7-50ug 50ug 现货 2600.00 2600
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熔点:
密度:
储存条件: -20℃

Cynomolgus / Rhesus macaque TNFR2 / CD120b / TNFRSF1B Protein, His Tag

分子别名(Synonym)

TNFRSF1B,CD120b,TBPII,TNF-R-II,TNF-R75,TNFBR,TNFR1B,TNFR2,TNFR80,p75,p75TNFR

表达区间及表达系统(Source)

Cynomolgus / Rhesus macaque TNFR2, His Tag  is expressed from human 293 cells . It contains AA Leu 23 - Asp 257.

Predicted N-terminus: Leu 23

Request for sequence

蛋白结构(Molecular Characterization)

This protein carries a polyhistidine tag at the C-terminus.

The protein has a calculated MW of 26.9 kDa. The protein migrates as 40-50 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.

内毒素(Endotoxin)

Less than 1.0 EU per μg by the LAL method.

纯度(Purity)

>90% as determined by SDS-PAGE.

制剂(Formulation)

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4. Normally trehalose is added as protectant before lyophilization.

Contact us for customized product form or formulation.

重构方法(Reconstitution)

Please see Certificate of Analysis for specific instructions.

For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

存储(Storage)

For long term storage, the product should be stored at lyophilized state at -20°C or lower.

Please avoid repeated freeze-thaw cycles.

This product is stable after storage at:

  1. -20°C to -70°C for 12 months in lyophilized state;

  2. -70°C for 3 months under sterile conditions after reconstitution.

 

电泳(SDS-PAGE)

Cynomolgus / Rhesus macaque TNFR2, His Tag (Cat. No. TN2-C52H7) SDS-PAGE gel

Cynomolgus / Rhesus macaque TNFR2, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 90%.

 

活性(Bioactivity)-ELISA

Biotinylated Human ELISA

Immobilized Biotinylated Human TNF-alpha, epitope tag free, primary amine labeling at 1 μg/mL (100 μL/well) on streptavidin  precoated (0.5 μg/well) plate can bind Cynomolgus / Rhesus macaque TNFR2, His Tagwith a linear range of 0.6-10 ng/mL (QC tested).

Protocol

Biotinylated Human  ELISA

Immobilized Cynomolgus / Rhesus macaque TNFR2, His Tag  at 5 μg/mL (100 μL/well) can bind Biotinylated Human TNF-alpha, epitope tag free, primary amine labelingwith a linear range of 0.3-10 ng/mL (Routinely tested).

Protocol

Biotinylated Human ELISA

Immobilized Cynomolgus / Rhesus macaque TNFR2, His Tag at 5 μg/mL (100 μL/well) can bind Biotinylated Human TNF-alpha, His,Avitag (active trimer) (MALS verified)with a linear range of 0.2-10 ng/mL (Routinely tested).

 

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